Background
Catalog Number:
CPTC-HPV 18 L1 VLP-1
Target Antigen:
HPV 18 L1 VLP
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/17/2018
Antigen Recognition(s):
Recombinant Full-length
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-HPV18 VLP-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Background
Catalog Number:
CPTC-HPV 18 L1 VLP-2
Target Antigen:
HPV 18 L1 VLP
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/17/2018
Antigen Recognition(s):
Recombinant Full-length
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-HPV18 VLP-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Background
Catalog Number:
CPTC-HPV 18 L1 VLP-3
Target Antigen:
HPV 18 L1 VLP
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/17/2018
Antigen Recognition(s):
Recombinant Full-length
Characterization Data [Compare Characterization Data]
Indirect ELISA
CPTC-HPV18 VLP-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Background
NCI Identification Number:
00260
Antigen Name:
HPV 18 L1 VLP
CPTC Name:
CPTC-HPV 18 L1 VLP
Aliases:
Function:
Virus like particles (VLPs) based on the L1 major capsid protein from HPV. Pooled human serum is used and serves as the current standard for some of the serological assays, which is used to create a curve from which results are extrapolated in arbitrary Units/ml.
VLPs are noninfectious protein capsids which can be produced by expressing the capsid protein in mammailian, yeast or insect cells. The recombinant L1 capsid protein assembles in into the 60-nm HPV VLP.
Reference for HPV-16 L1 plasmid and protein origins: Enhancement of Capsid Gene Expression: Leder, C. el al. "Preparing the Human Papillomavirus Type 16 Major Structural Gene L1 for DNA Vaccination Purposes" J. Virology (2001) 75:9201-9209.
Chromosomal Localization:
Expression System:
Mammalian
Accession Number:
000
UniProt Accession Number:
DNA Source:
N/A
Immunogen:
Native Protein
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
MALWRPSDNTVYLPPPSVARVVNTDDYVTRTSIFYHAGSSRLLTVGNPYF
RVPAGGGNKQDIPKVSAYQYRVFRVQLPDPNKFGLPDTSIYNPETQRLVW
ACAGVEIGRGQPLGVGLSGHPFYNKLDDTESSHAATSNVSEDVRDNVSVD
YKQTQLCILGCAPAIGEHWAKGTACKSRPLSQGDCPPLELKNTVLEDGDM
VDTGYGAMDFSTLQDTKCEVPLDICQSICKYPDYLQMSADPYGDSMFFCL
RREQLFARHFWNRAGTMGDTVPQSLYIKGTGMRASPGSCVYSPSPSGSIV
TSDSQLFNKPYWLHKAQGHNNGVCWHNQLFVTVVDTTRSTNLTICASTQS
PVPGQYDATKFKQYSRHVEEYDLQFIFQLCTITLTADVMSYIHSMNSSIL
EDWNFGVPPPPTTSLVDTYRFVQSVAITCQKDAAPAENKDPYDKLKFWNV
DLKEKFSLDLDQYPLGRKFLVQAGLRRKPTIGPRKRSAPSATTSSKPAKR
VRVRARK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1
Last Updated:
05/17/2018
Links
Characterization Data
SOPs
No SOPs available.
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