PRINT

Gelsolin

Antibody: CPTC-Gelsolin-1
Background

Catalog Number:

CPTC-Gelsolin-1

RRID:

AB_2263829

Target Antigen:

Gelsolin

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

05/23/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on normal endometrium tissue core using CPTC-Gelsolin-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate). Click image to enlarge

CPTC-Gelsolin-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on normal endometrium tissue core using CPTC-Gelsolin-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (178.5 KB)

CPTC-Gelsolin-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Gelsolin-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-Gelsolin-1 CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-Gelsolin-1 Protein Array

Result: Positive

Protein Array in which CPTC-Gelsolin-1 CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-Gelsolin-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Gelsolin-1 as primary Ab against Ag 10594 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Gelsolin-1 Western Blot

Result: Positive

Western Blot using CPTC-Gelsolin-1 as primary Ab against Ag 10594 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antibody: CPTC-Gelsolin-2
Background

Catalog Number:

CPTC-Gelsolin-2

RRID:

AB_2617260

Target Antigen:

Gelsolin

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Download This table summarizes the results of the pairing experiment with Gelsolin antibodies. (769.0 KB)

SPR pairing results

Result: Positive

This table summarizes the results of the pairing experiment with Gelsolin antibodies.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for Gelsolin-2 using Biacore SPR method Click image to enlarge

CPTC-Gelsolin-2 SPR

Result: Positive

Kinetic titration data for Gelsolin-2 using Biacore SPR method


Characterization SOP Files

  IHC NCI60

CPTC-Gelsolin-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-Gelsolin-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-Gelsolin-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Gelsolin-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-Gelsolin-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2). Click image to enlarge

CPTC-Gelsolin-2 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-Gelsolin-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Gelsolin-2 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Gelsolin-2 Western blot

Result: Positive

Western Blot using CPTC-Gelsolin-2 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antibody: CPTC-Gelsolin-3
Background

Catalog Number:

CPTC-Gelsolin-3

RRID:

AB_2617261

Target Antigen:

Gelsolin

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Download This table summarizes the results of the pairing experiment with Gelsolin antibodies. (769.0 KB)

SPR pairing results

Result: Positive

This table summarizes the results of the pairing experiment with Gelsolin antibodies.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for Gelsolin-3 antibody using Biacore SPR method Click image to enlarge

CPTC-Gelsolin-3 SPR

Result: Positive

Kinetic titration data for Gelsolin-3 antibody using Biacore SPR method


Characterization SOP Files

  IHC NCI60

CPTC-Gelsolin-3 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC NCI60

CPTC-Gelsolin-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-Gelsolin-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Gelsolin-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-Gelsolin-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2). Click image to enlarge

CPTC-Gelsolin-3 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-Gelsolin-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Gelsolin-3 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Gelsolin-3 Western blot

Result: Positive

Western Blot using CPTC-Gelsolin-3 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antibody: CPTC-Gelsolin-4
Background

Catalog Number:

CPTC-Gelsolin-4

RRID:

AB_2617262

Target Antigen:

Gelsolin

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR
Download This table summarizes the results of the pairing experiment with Gelsolin antibodies. (769.0 KB)

SPR pairing results

Result: Positive

This table summarizes the results of the pairing experiment with Gelsolin antibodies.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Kinetic titration data for Gelsolin-4 antibody using Biacore SPR method Click image to enlarge

CPTC Gelsolin-4 SPR

Result: Positive

Kinetic titration data for Gelsolin-4 antibody using Biacore SPR method


Characterization SOP Files

  IHC NCI60

CPTC-Gelsolin-4 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-Gelsolin-4 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-Gelsolin-4 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-Gelsolin-4 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-Gelsolin-4 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2). Click image to enlarge

CPTC-Gelsolin-4 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-Gelsolin-4 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 3). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 4) and recombinant Ag Gelsolin (NCI 10594) in (lane 2).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Gelsolin-4 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Gelsolin-4 Western blot

Result: Positive

Western Blot using CPTC-Gelsolin-4 as primary Ab against Gelsolin (rAg 10594) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

[top]

Antigen: Gelsolin

Background

NCI Identification Number:

10594

Antigen Name:

Gelsolin

CPTC Name:

CPTC-Gelsolin

Aliases:

amyloidosis, Finnish type; GSN; ADF; AGEL; Brevin; DKFZp313L0718; OTTHUMP00000022011; OTTHUMP00000162202

Function:

The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments
to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.

Chromosomal Localization:

9q33

Expression System:

E. Coli

Accession Number:

BC026033

UniProt Accession Number:

P06396

DNA Source:

HIP:HsCD00005767

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

115405

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAATASRGASQAGAPQGRVPEARPNSMVVEHPEFLKAGKEPGLQIWRVE
KFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDE
SGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVAS
GFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNI
HQWCGSNSNRYERLKATQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVL
GPKPALPAGTEDTAKEDAANRKLAKLYKVSNGAGTMSVSLVADENPFAQG
ALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDFITKMDYPKQ
TQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDA
ATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSY
IILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQS
RVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAG
ATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRV
LRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSN
KIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEAL
TSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLD
RAMAELAA

Native Sequence:

MAPHRPAPALLCALSLALCALSLPVRAATASRGASQAGAPQGRVPEARPN
SMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKT
VQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQ
GFESATFLGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRA
TEVPVSWESFNNGDCFILDLGNNIHQWCGSNSNRYERLKATQVSKGIRDN
ERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANRKLAKL
YKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQA
NTEERKAALKTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPD
QTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIW
RIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQ
DEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGG
TSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPS
AAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAA
YRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVML
LDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQG
FEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA

Calculated Isoelectric Point:

5.71

Molecular Weight:

83231

Last Updated:

09/01/2008

Links

Characterization Data

Gel

Click to enlarge image PAGE of Ag 10594 with molecular weight standards in lane 1
Click image to enlarge

Ag 10594 PAGE

PAGE of Ag 10594 with molecular weight standards in lane 1

[top]

SOPs

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]