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Glutathione S Transferase Mu3

Antibody: CPTC-GST Mu3-1
Background

Catalog Number:

CPTC-GST Mu3-1

RRID:

AB_10567133

Target Antigen:

Glutathione S Transferase Mu3

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (134.5 KB)

CPTC-GSTMu3-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Human assay: A-431 fixed with PFA, dilution: 1:100 Human assay: U-2 OS fixed with PFA, dilution: 1:100 Human assay: U-251 MG fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-GSTMu3-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Human assay: A-431 fixed with PFA, dilution: 1:100
Human assay: U-2 OS fixed with PFA, dilution: 1:100
Human assay: U-251 MG fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu3-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-GSTMu3-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:250. Click image to enlarge

CPTC-GSTMu3-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:250.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GSTMu3-1 as primary Ab against Ag 10080 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu3-1 Western blot

Result: Positive

Western Blot using CPTC-GSTMu3-1 as primary Ab against Ag 10080 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-GST Mu3-2
Background

Catalog Number:

CPTC-GST Mu3-2

RRID:

AB_10572300

Target Antigen:

Glutathione S Transferase Mu3

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (119.9 KB)

CPTC-GSTMu3-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu3-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-GSTMu3-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-GSTMu3-2 NCI60 Protein Array

Result: Positive

Protein Array in which CPTC-GSTMu3-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GSTMu3-2 as primary Ab against Ag 10080 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu3-2 Western blot

Result: Positive

Western Blot using CPTC-GSTMu3-2 as primary Ab against Ag 10080 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-GST Mu3-3
Background

Catalog Number:

CPTC-GST Mu3-3

RRID:

AB_2266427

Target Antigen:

Glutathione S Transferase Mu3

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Cross Reactivity Data
Click to enlarge image This table shows the cross reactivity by indirect ELISA with the GST group of antigens. Click image to enlarge

Cross Reactivity Data

Result: Positive

This table shows the cross reactivity by indirect ELISA with the GST group of antigens.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (121.9 KB)

CPTC-GSTMu3-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-GSTMu3-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-GSTMu3-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-GSTMu3-3 as primary Ab against Ag 10080 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-GSTMu3-3 Western blot

Result: Positive

Western Blot using CPTC-GSTMu3-3 as primary Ab against Ag 10080 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Glutathione S Transferase Mu3
Background

NCI Identification Number:

10080

Antigen Name:

Glutathione S Transferase Mu3

CPTC Name:

CPTC-GST Mu3

Aliases:

GSTM3; EC 2.5.1.18; GST5; GSTB; GSTM3-3; GTM3; MGC3310; MGC3704; OTTHUMP00000013355; hGSTM3-3

Function:

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. Mutations of this class mu gene have been linked with a slight increase in a number of cancers, likely due to exposure with environmental toxins. Alternative splicing results in multiple transcript variants. Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

Chromosomal Localization:

1q13.3

Expression System:

E. Coli

Accession Number:

BC000088.2

UniProt Accession Number:

P21266

DNA Source:

HsCD00003148

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

37150

Buffers:

PBS

Expressed Sequence:

SNAMSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDR
SQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEE
KIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLG
KFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEK
IAAYLQSDQFCKMPINNKMAQWGNKPVC

Native Sequence:

MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQW
LDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIR
VDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFS
WFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAA
YLQSDQFCKMPINNKMAQWGNKPVC

Calculated Isoelectric Point:

5.37

Molecular Weight:

26832

Last Updated:

08/22/2020

Links

Characterization Data

SOPs

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