Growth Factor Receptor-Bound Protein 2 Peptide 1


Background

Catalog Number:

CPTC-GRB2-1

Target Antigen:

Growth Factor Receptor-Bound Protein 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/31/2015

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-GRB2-1 as primary Ab against full-length recombinant Ag 10390 (lane 2). Also included are molecular wt. standards (lane 1) and the GRB2-1 Ab as positive control (lane 3). Click image to enlarge

CPTC-GRB2-1 Western blot

Western Blot using CPTC-GRB2-1 as primary Ab against full-length recombinant Ag 10390 (lane 2). Also included are molecular wt. standards (lane 1) and the GRB2-1 Ab as positive control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-GRB2-1 peptide ELISA

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

Background

NCI Identification Number:

00026

Antigen Name:

Growth Factor Receptor-Bound Protein 2 Peptide 1

CPTC Name:

CPTC-GRB2 Peptide 1

Keywords:

NCKAP2; Protein Ash; ASH; SH2/SH3 Adapter GRB2; EGFRBP-GRB2; Grb3-3; HT027; MST084; MSTP084; Abundant SRC Homology; Epidermal Growth Factor Receptor-Binding; Protein GRB2; Growth Factor Receptor Bound Protein 3; Adapter Protein GRB2

Function:

The protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3
domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras
signaling pathway.

Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Chromosomal Localization:

17q24-q25

Expression System:

NCBI Accession Number:

P62993

Swiss Prot Accession Number:

P62993

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

FGNDVQHFK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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