Fascin


Background

Catalog Number:

CPTC-Fascin-1

Target Antigen:

Fascin

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

12/15/2011

Antigen Recognition(s):

recombinant full-length

External Links
Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-Fascin-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Fascin-1 Western Blot

Western Blot using CPTC-Fascin-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Fascin-1 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Background

Catalog Number:

CPTC-Fascin-2

Target Antigen:

Fascin

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

03/07/2013

Antigen Recognition(s):

recombinant full-length

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-Fascin-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Fascin-2 Western Blot

Western Blot using CPTC-Fascin-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Fascin-2 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-Fascin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-Fascin-2 Protein Array

Protein Array in which CPTC-Fascin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).

  IHC NCI60

CPTC-Fascin-2 IHC NCI60

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Click to view IHC NCI60 Cell Line Array example
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IHC Cell Line Images

IHC NCI60 Cell Line Array example
  IHC Tissue

CPTC-Fascin-2 IHC Tissue

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.

Background

Catalog Number:

CPTC-Fascin-3

Target Antigen:

Fascin

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/26/2013

Antigen Recognition(s):

recombinant full-length

Purchase
Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Fascin-3 Western Blot

Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Fascin-3 Indirect ELISA

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)

Background

NCI Identification Number:

10411

Antigen Name:

Fascin

CPTC Name:

CPTC-Fascin

Keywords:

FSCN1; FAN1; FLJ38511; Fascin; HSN; SNL; p55

Function:

Chromosomal Localization:

7p22

Expression System:

E. Coli

NCBI Accession Number:

BC007948

Swiss Prot Accession Number:

Q16658

DNA Source:

HIP:HsCD00004052

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

68153

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWT
LEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHD
DGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSV
TRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLR
HDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATK
VGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLE
IDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITL
RASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGC
RKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDT
PVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Native Sequence:

MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQ
PPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGR
WSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRK
RYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDG
RLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGK
DELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR
DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRAS
NGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKV
TGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVD
FFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Calculated Isoelectric Point:

6.83

Molecular Weight:

54802

Last Updated:

04/09/2013

Links

Characterization Data

Gel

Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Click image to enlarge

CPTC-Fascin (rAg 10411) PAGE

PAGE of Fascin (rAg 10411) with molecular weight standards in lane 1.

SOPs

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