Background
Catalog Number:
CPTC-FOXO3-3
Target Antigen:
Forkhead Box O3 Peptide 3
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
03/25/2021
Antigen Recognition(s):
Peptide, Recombinant Full-length
Characterization Data [Compare Characterization Data]
Western Blot - Recombinant Protein or Peptide
CPTC-FOXO3-3 Western Blot (Recombinant Protein)
Result: Positive
Western blot using CPTC-FOXO3-3 as primary antibody against human FOXO3 recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 71.1 kDa.
Blot was developed using enhanced chemiluminescence (ECL).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-FOXO3-3 Western Blot Cell Lysates
Result: Negative
Western Blot using CPTC-FOXO3-3 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). All cell lines are negative. Expected molecular weights – 71.2 kDa and 48.4kDa.
Characterization SOP Files
Background
NCI Identification Number:
00458
Antigen Name:
Forkhead Box O3 Peptide 3
CPTC Name:
CPTC-FOXO3 Peptide 3
Aliases:
Forkhead Box O3; Forkhead In Rhabdomyosarcoma-Like 1; Forkhead Box Protein O3; AF6q21; FKHRL1; FOXO3A; FOXO2; Forkhead, Drosophila, Homolog Of, In Rhabdomyosarcoma-Like 1; Forkhead Homolog (Rhabdomyosarcoma) Like 1; Forkhead Box O3A; AF6q21 Protein; FKHRL1P2; FOXO3
Function:
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.FOXO3 (Forkhead Box O3) is a Protein Coding gene. Diseases associated with FOXO3 include Chromosome 6Q Deletion and Rhabdomyosarcoma. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and RET signaling. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and protein kinase binding. An important paralog of this gene is FOXO1.ranscriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy (PubMed:10102273, PubMed:16751106, PubMed:21329882). Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins (By similarity). Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress (PubMed:10102273, PubMed:16751106). Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation (PubMed:21329882). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription (PubMed:23283301). In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity).
Chromosomal Localization:
6q21
Accession Number:
NP_001446.1
UniProt Accession Number:
O43524
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
AVS(ph)MDNSNKYTK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1320
Last Updated:
10/23/2020
Links
- EnsEMBL - EnsEMBL FOXO3
- Entrez - Entrez FOXO3
- Uniprot - Uniprot O43524
Characterization Data
SOPs
No SOPs available.
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