Flap Structure-Specific Endonuclease 1 Peptide 1


Background

Catalog Number:

CPTC-FEN1-1

Target Antigen:

Flap Structure-Specific Endonuclease 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

External Links
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-FEN1-1

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-FEN1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3230) Click image to enlarge

CPTC-FEN1-1 IMRM

Immuno-MRM chromatogram of CPTC-FEN1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3230)

Background

NCI Identification Number:

00027

Antigen Name:

Flap Structure-Specific Endonuclease 1 Peptide 1

CPTC Name:

CPTC-FEN1 Peptide 1

Keywords:

FEN-1; MF1; RAD2; Maturation Factor 1; DNase IV; Flap Endonuclease 1; Maturation Factor-1; EC 3.1.; HFEN-1

Function:

The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Chromosomal Localization:

11q12

Expression System:

NCBI Accession Number:

NP_004102.1

Swiss Prot Accession Number:

P39748

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SIEEIVR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

770

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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