Ezrin Peptide 1


Background

Catalog Number:

CPTC-Ezrin-2

Target Antigen:

Ezrin Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/01/2015

Antigen Recognition(s):

Characterization Data [Compare Characterization Data]
  Western Blot
Click to enlarge image Western Blot using CPTC-EZRIN-2 as primary Ab against full-length recombinant Ag 10474 (lane 2). Also included are molecular wt. standards (lane 1) and the EZRIN-2 Ab as positive control (lane 3). Click image to enlarge

CPTC-EZRIN-2 WESTERN BLOT

Western Blot using CPTC-EZRIN-2 as primary Ab against full-length recombinant Ag 10474 (lane 2). Also included are molecular wt. standards (lane 1) and the EZRIN-2 Ab as positive control (lane 3).

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EZRIN-2 peptide ELISA

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.

Characterization SOP Files

Background

NCI Identification Number:

00047

Antigen Name:

Ezrin Peptide 1

CPTC Name:

CPTC-Ezrin Peptide 1

Keywords:

EZR; VIL2; Villin 2 (Ezrin); CVL; P81; CVIL; Cytovillin 2; Villin-2; Cytovillin

Function:

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial
cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis

Chromosomal Localization:

6q25.3

Expression System:

NCBI Accession Number:

NP_001104547.1

Swiss Prot Accession Number:

P15311

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SGYLSSER

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

880

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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