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Ezrin Peptide 1

Antibody: CPTC-Ezrin-2
Background

Catalog Number:

CPTC-Ezrin-2

RRID:

AB_2888949

Target Antigen:

Ezrin Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (382.8 KB)

CPTC-EZRIN-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-Ezrin-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-712) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-Ezrin-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-Ezrin-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-712)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EZRIN-2 peptide ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-EZRIN-2 as primary Ab against full-length recombinant Ag 10474 (lane 2). Also included are molecular wt. standards (lane 1) and the EZRIN-2 Ab as positive control (lane 3). Click image to enlarge

CPTC-EZRIN-2 WESTERN BLOT

Result: Positive

Western Blot using CPTC-EZRIN-2 as primary Ab against full-length recombinant Ag 10474 (lane 2). Also included are molecular wt. standards (lane 1) and the EZRIN-2 Ab as positive control (lane 3).


Characterization SOP Files

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Antigen: Ezrin Peptide 1
Background

NCI Identification Number:

00047

Antigen Name:

Ezrin Peptide 1

CPTC Name:

CPTC-Ezrin Peptide 1

Aliases:

EZR; VIL2; Villin 2 (Ezrin); CVL; P81; CVIL; Cytovillin 2; Villin-2; Cytovillin

Function:

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial
cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis

Chromosomal Localization:

6q25.3

Accession Number:

NP_001104547.1

UniProt Accession Number:

P15311

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

SGYLSSER

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

880

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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