Background
Catalog Number:
CPTC-Ezrin-1
RRID:
AB_2100318
Target Antigen:
Ezrin (p81)
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-Ezrin-1
External Links
- Human Protein Atlas CPTC-Ezrin-1
Publications
- Discovery of Novel Disease-specific and Membrane-associated Candidate Markers in a Mouse Model of Multiple Sclerosis (PMID: 24361864)
- Ezrin activation by LOK phosphorylation involves a PIP2-dependent wedge mechanism (PMID: 28430576)
- Human placental-derived stem cell therapy ameliorates experimental necrotizing enterocolitis (PMID: 33566727)
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (110.7 KB)
CPTC-Ezrin-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-Ezrin-1 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-Erzin-1 for NCI60 Cell Line Array at titer 1:4000
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Characterization SOP Files
IHC Tissue
CPTC-Ezrin-1 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-Ezrin-1. Titer: 1:4000
Characterization SOP Files
Immunofluorescence - HPA
CPTC-Ezrin-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane. uman assay: A-431 fixed with PFA, dilution: 1:2000
Human assay: U-2 OS fixed with PFA, dilution: 1:2000
Human assay: U-251 MG fixed with PFA, dilution: 1:2000
Characterization SOP Files
Indirect ELISA
CPTC-Ezrin-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-Ezrin-1 NCI60 Protein Array
Result: Positive
Protein Array in which CPTC-Erzin-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-Ezrin-1 Western Blot
Result: Positive
Western Blot using CPTC-Erzin-1 as primary Ab against Ag 10474 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-Ezrin-1 Cell Lysate Western blot
Result: Positive
Western Blot using CPTC-Ezrin-1 as primary Ab against cell lysate from OVCAR-3 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10474
Antigen Name:
Ezrin (p81)
CPTC Name:
CPTC-Ezrin
Aliases:
EZR; CVIL; CVL; Cytovillin; DKFZp762H157; FLJ26216; MGC1584; OTTHUMP00000017506; OTTHUMP00000017507; VIL2; Villin-2; ezrin; p81
Function:
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene.
Alternatively spliced variants have also been described for this gene. Probably involved in connections of major cytoskeletal structures to the plasma membrane.
In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Chromosomal Localization:
P15311
Expression System:
E. Coli
Accession Number:
BC013903
UniProt Accession Number:
P15311
DNA Source:
HIP:HsCD00041738
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
66873
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGL
HYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDI
TQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYL
SSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQD
LEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRN
ISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKP
DTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEEL
MLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAAL
RAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQH
RAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPT
GYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKR
THNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL
Native Sequence:
MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYV
DNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQK
LFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSE
RLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEM
YGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISF
NDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTI
EVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLR
LQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAK
EELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAK
EAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYS
AELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHN
DIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL
Calculated Isoelectric Point:
5.94
Molecular Weight:
69685
Last Updated:
09/01/2008
Links
- Entrez - Entrez Ezrin
- Uniprot - Uniprot P15311
Characterization Data
Gel
Click image to enlarge
Ag 10474 PAGE
PAGE of Ag 10474 (with molecular weight standards in lane 1)
Characterization SOP Files
SOPs
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