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ERBB Receptor Feedback Inhibitor 1 Peptide 1

Antibody: CPTC-ERRFI1-1
Background

Catalog Number:

CPTC-ERRFI1-1

Target Antigen:

ERBB Receptor Feedback Inhibitor 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Phosphorylation

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (333.9 KB)

CPTC-ERRFI1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-ERRFI1-1 against synthetic peptide VSSTHY-pY-LLPERPPYLDK. The antibody was not able to pull down the other two peptides VSSTH-pY-pY-LLPERPPYLDK and VSSTH-pY-YLLPERPPYLDK. Click image to enlarge

CPTC-ERRFI1-1 iMRM against synthetic peptide

Result: Positive

iMRM screening of CPTC-ERRFI1-1 against synthetic peptide VSSTHY-pY-LLPERPPYLDK. The antibody was not able to pull down the other two peptides VSSTH-pY-pY-LLPERPPYLDK and VSSTH-pY-YLLPERPPYLDK.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-ERRFI1-1. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide VSSTHYpYLLPERPPYLDK (CPTC-ERRF1 Peptide 1). Click image to enlarge

CPTC-ERRFI1-1 iMRM (endogenous peptide)

Result: Positive

iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-ERRFI1-1. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide VSSTHYpYLLPERPPYLDK (CPTC-ERRF1 Peptide 1).


Characterization SOP Files

  Immunofluorescence

CPTC-ERRFI1-1

Result: Negative

Immunofluorescence staining of human cell line A549 with CPTC-ERRFI1-1 Ab shows no localization of ERRFI1 protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-ERRFI1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot usnig CPTC-ERRFI1-1 against the ERRFI1 Overexpressed Cell Lysate. Expected MW is about 50 KDa Click image to enlarge

CPTC-ERRFI1-1 Overexpressed Cell Lysate Western Blot

Result: Positive

Western Blot usnig CPTC-ERRFI1-1 against the ERRFI1 Overexpressed Cell Lysate. Expected MW is about 50 KDa


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-ERFFI1-1 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 50 KDa. Positive for A549 lysates, inconclusive/negative results for the other cell lysates. Molecular weight standards are also included (lane 1). ECL detection. Click image to enlarge

CPTC-ERRFI1-1 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-ERFFI1-1 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 50 KDa. Positive for A549 lysates, inconclusive/negative results for the other cell lysates. Molecular weight standards are also included (lane 1). ECL detection.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-ERRFI1-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-ERRFI1-1 as a primary antibody against cell lysates. ERRFI1 protein was not detected and therefore no expression graphs were generated.


Characterization SOP Files

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Antibody: CPTC-ERRFI1-2
Background

Catalog Number:

CPTC-ERRFI1-2

Target Antigen:

ERBB Receptor Feedback Inhibitor 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (469.7 KB)

CPTC-ERRFI1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-ERRFI1-2 against the synthetic peptide VSSTHY-pY-LLPERPPYLDK. The antibody is also able to pull down the other two peptides VSSTH-pY-pY-LLPERPPYLDK and VSSTH-pY-YLLPERPPYLDK. Click image to enlarge

CPTC-ERRFI1- 2 iMRM against synthetic peptide

Result: Positive

iMRM screening of CPTC-ERRFI1-2 against the synthetic peptide VSSTHY-pY-LLPERPPYLDK. The antibody is also able to pull down the other two peptides VSSTH-pY-pY-LLPERPPYLDK and VSSTH-pY-YLLPERPPYLDK.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-ERRFI1-2. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide VSSTHYpYLLPERPPYLDK (CPTC-ERRF1 Peptide 1). Click image to enlarge

CPTC-ERRFi1-2 iMRM (endogenous peptide)

Result: Positive

iMRM data obtaiend form the incubation of the cell lysate H3255 digest with clone CPTC-ERRFI1-2. Data were acquired on the nano-chip-LC using a 1260 Infinity Series HPLC-Chip cube interface (Agilent, Palo Alto, CA) coupled to a 6495-triple quadrupole mass spectrometer (Agilent). A large capacity chip system (G4240-62010) consisting of a 160 nl enrichment column and a 150 mm*75 um analytical column (Zorbax 300SB-C18, 5 um, 30 A pore size) was used. The clone is able to capture the endogenous target peptide VSSTHYpYLLPERPPYLDK (CPTC-ERRF1 Peptide 1).


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line A549 with CPTC-ERRFI1-2 Ab shows localization to the nucleus and cytosol. Click image to enlarge

CPTC-ERRFI1-2 Immunofluorescence

Result: Positive

Immunofluorescence staining of human cell line A549 with CPTC-ERRFI1-2 Ab shows localization to the nucleus and cytosol.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (469.7 KB)

CPTC-ERRFI1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-ERRFI1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-ERRFI1-2 against the ERRFI1 over-expressed lysate. Expected MW is about 50 KDa. Click image to enlarge

CPTC-ERRFI1-2 against Over-expressed Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-ERRFI1-2 against the ERRFI1 over-expressed lysate. Expected MW is about 50 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-ERFFI1-2 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 50 KDa. Positive for A549 lysates, inconclusive/negative results for the other cell lysates. Molecular weight standards are also included (lane 1). ECL detection. Click image to enlarge

CPTC-ERRFI1-2 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-ERFFI1-2 as primary antibody against cell lysates OVCAR3, HCT116, PC-3, MBA-MD-231 and A549 (lane 2) with expected MW of 50 KDa. Positive for A549 lysates, inconclusive/negative results for the other cell lysates. Molecular weight standards are also included (lane 1). ECL detection.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-ERRFI1-2 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-ERRFI1-2 as a primary antibody against cell lysates. ERRFI1 protein was not detected and therefore no expression graphs were generated.


Characterization SOP Files

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Antigen: ERBB Receptor Feedback Inhibitor 1 Peptide 1
Background

NCI Identification Number:

00341

Antigen Name:

ERBB Receptor Feedback Inhibitor 1 Peptide 1

CPTC Name:

CPTC-ERRFI1 Peptide 1

Aliases:

ERBB Receptor Feedback Inhibitor 1; MIG-6; Mitogen-Inducible Gene 6 Protein; GENE-33; MIG6; RALT; Receptor-Associated Late Transducer; ERRFI1

Function:

ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al., 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33, which is induced during cell stress and mediates cell signaling (Makkinje et al., 2000 [PubMed 10749885]; Fiorentino et al., 2000 [PubMed 11003669]).ERRFI1 (ERBB Receptor Feedback Inhibitor 1) is a Protein Coding gene. Diseases associated with ERRFI1 include Endometrial Disease and Progesterone Resistance. Among its related pathways are EGF/EGFR Signaling Pathway and Tyrosine Kinases / Adaptors. Gene Ontology (GO) annotations related to this gene include protein kinase binding and kinase binding.Negative regulator of EGFR signaling in skin morphogenesis. Acts as a negative regulator for several EGFR family members, including ERBB2, ERBB3 and ERBB4. Inhibits EGFR catalytic activity by interfering with its dimerization. Inhibits autophosphorylation of EGFR, ERBB2 and ERBB4. Important for normal keratinocyte proliferation and differentiation. Plays a role in modulating the response to steroid hormones in the uterus. Required for normal response to progesterone in the uterus and for fertility. Mediates epithelial estrogen responses in the uterus by regulating ESR1 levels and activation. Important for regulation of endometrium cell proliferation. Important for normal prenatal and perinatal lung development (By similarity).

Chromosomal Localization:

1p36.23

Accession Number:

NP_061821.1

UniProt Accession Number:

Q9UJM3

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VSSTHY-pY-LLPERPPYLDK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1980

Last Updated:

08/25/2020

Links

Characterization Data

SOPs

No SOPs available.

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