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EPH Receptor B4

Antibody: CPTC-EPHB4-1
Background

Catalog Number:

CPTC-EPHB4-1

RRID:

AB_2617253

Target Antigen:

EPH Receptor B4

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

11/06/2019

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC NCI60

CPTC-EPHB4-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-EPHB4-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EPHB4-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-EPHB4-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-EPHB4-1 as primary Ab against EPHB4 (rAg 10657) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-EPHB4-1 Western blot

Result: Positive

Western Blot using CPTC-EPHB4-1 as primary Ab against EPHB4 (rAg 10657) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-EPHB4-2
Background

Catalog Number:

CPTC-EPHB4-2

RRID:

AB_2617254

Target Antigen:

EPH Receptor B4

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/06/2019

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC NCI60

CPTC-EPHB4-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-EPHB4-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-EPHB4-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-EPHB4-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-EPHB4-2 as primary Ab against EPHB4 (Ag 10657) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-EPHB4-2 Western blot

Result: Positive

Western Blot using CPTC-EPHB4-2 as primary Ab against EPHB4 (Ag 10657) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: EPH Receptor B4
Background

NCI Identification Number:

10657

Antigen Name:

EPH Receptor B4

CPTC Name:

CPTC-EPHB4

Aliases:

EPH receptor B4; HTK; MYK1; Hepatoma transmembrane kinase; Tyrosine-protein kinase TYRO11; TYRO11; EC 2.7.10.1; EPHB4; ephrin type-B receptor 4; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; tyrosine-protein kinase receptor HTK; EC 2.7.10

Function:

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous
system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class,
which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are
transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their
extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent
cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Ephrins are a family of receptor tyrosine kinases (RTKs) that influence cell migration. In conjunction with the ephrin ligands, ephrin receptors are involved in a variety of developmental events. EphB4 is overexpressed in many tumor types, including breast and prostate cancer.

Chromosomal Localization:

7q22

Expression System:

E. Coli

Accession Number:

BC019307.1

UniProt Accession Number:

P54760

DNA Source:

DNASU - HsCD00021508

Immunogen:

Recombinant Domain

Vector Name:

pMCSG7

Extinction Coefficient:

11460

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNAPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPS
SVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLD

Native Sequence:

MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDE
EQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSL
PRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKR
PGAEATGKVNVRTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQL
TVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPV
TGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSA
VCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESG
GREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDF
TYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSL
SLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRG
ASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVV
LVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPN
EAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLK
GGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGA
LDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNS
NLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSA
SDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLH
QLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLD
QRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRI
GVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

Calculated Isoelectric Point:

9.52

Molecular Weight:

10723

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of EPHB4 (rAg 10657) with molecular weight standards in lane 1
Click image to enlarge

CPTC-EPHB4 PAGE

PAGE of EPHB4 (rAg 10657) with molecular weight standards in lane 1

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SOPs

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