Background
Catalog Number:
CPTC-EGFR-7
RRID:
AB_2722051
Target Antigen:
Epidermal Growth Factor Receptor Peptide 3
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
07/19/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-EGFR-7
External Links
- Human Protein Atlas CPTC-EGFR-7
Publications
- A dataset describing a suite of novel antibody reagents for the RAS signaling network (PMID: 31467290)
- Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling (PMID: 34671754)
Characterization Data [Compare Characterization Data]
Flow Cytometry
CPTC-EGFR-7 (Flow Cytometry)
Result: Negative
Flow cytometric analysis of epidermal growth factor receptor (EGFR) cell surface expression using CPTC-EGFR-7 antibody. H-226 cells were blocked, stained with CPTC-EGFR-7 (solid green) or concentration-matched mouse IgG1 isotype control (dashed green) antibodies and then fixed. HL-60 cells were blocked, stained with CPTC-EGFR-7 (solid blue) or concentration-matched mouse IgG1 isotype control (dashed blue) antibodies, and then fixed. A BV421 conjugated goat anti-mouse IgG was used as a secondary antibody. All data were analyzed using FlowJo.
Characterization SOP Files
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (529.5 KB)
CPTC-EGFR-7 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-EGFR-7 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-EGFR-7antibody with CPTC-EGFR peptide 3 (NCI ID#00114) as target
Immunofluorescence
CPTC-EGFR-7 Immunofluorescence
Result: Negative
Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-EGFR-7 antibody. EGFR protein expression was not detected.
Characterization SOP Files
Immunofluorescence
CPTC-EGFR-7 Immunofluorescence
Result: Negative
Immunofluorescence staining using CPTC-EGFR-7 as primary antibody against HL-60 and NCI H226 cells. EGFR protein expression shows no localization in either cell lines.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-EGFR-7 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to plasma membrane. Human assay:
THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100
Characterization SOP Files
Western Blot - Over-expressed transient protein in cell lysate
CPTC-EGFR-7 Western Blot
Result: Positive
Western Blot using CPTC-EGFR-7 as primary Ab against EGFR over-expressed lysate.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-EGFR-7 - SW recombinant protein
Result: Positive
SW using CPTC-EGFR-7 as primary antibody against the over-expressed lysate of EGFR. The antibody is able to recognize the recombinant protein.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-EGFR-7 Cell Lysate Western Blot
Result: Negative
Western Blot using CPTC-EGFR-7 as primary Ab against he while lysates of A498, ACHN, H226, H322M, CCRF-CEM, and HL-60. the antibody was not able to recognize endogenous EGFR in any of the tested cell lysates.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-EGFR-7 Single Cell Western Blot (Cell Lysate)
Result: Negative
Single cell western blot using CPTC-EGFR-7 as a primary antibody against HL-60 and NCI H226 cell lysates. EGFR protein expression was not detected in either cell lysates.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-EGFR-7 - SW cell lysates
Result: Negative
SW using CPTC-EGFR-7 as primary antibody against the whole lysates of A498, ACHN, H226, H322M, CCRF-CEM and HL-60. The antibody is not able to recognize the endogenous protein in any of the tested lysates.
Characterization SOP Files
Background
NCI Identification Number:
00114
Antigen Name:
Epidermal Growth Factor Receptor Peptide 3
CPTC Name:
CPTC-EGFR Peptide 3
Aliases:
Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB; HER1; ERBB1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EC 2.7.10; NISBD2; PIG61; MENA
Function:
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
EGFR (Epidermal Growth Factor Receptor) is a Protein Coding gene. Diseases associated with EGFR include lung cancer and inflammatory skin and bowel disease, neonatal, 2. Among its related pathways are PI3K-Akt signaling pathway and PI-3K cascade. GO annotations related to this gene include identical protein binding and chromatin binding. An important paralog of this gene is TNK1.
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.
The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase of the ErbB family. Four members of the ErbB family have been identified; EGFR (ErbB1, HER1), ErbB2 (HER2), ErbB3 (HER3) and ErbB4 (HER4). EGFR signaling is initiated by ligand binding to the extracellular ligand binding domain. This initiates receptor homo-/hetero-dimerization and autophosphorylation by the intracellular kinase domain, resulting in receptor activation. Following activation, phosphorylation of cytoplasmic substrates occurs and a signaling cascade is initiated that drives many cellular responses, including changes in gene expression, cytoskeletal rearrangement, anti-apoptosis and increased cell proliferation.
Chromosomal Localization:
7p12
Accession Number:
NP_005219.2
UniProt Accession Number:
P00533
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
NLQEILHGAVR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1210
Last Updated:
10/01/2019
Links
- Entrez - Entrez EGFR
- EnsEMBL - EnsEMBL EGFR
- Uniprot - Uniprot P00533
Characterization Data
SOPs
No SOPs available.
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