Epidermal Growth Factor Receptor Peptide 4


Background

Catalog Number:

CPTC-EGFR-1

Target Antigen:

Epidermal Growth Factor Receptor Peptide 4

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

04/13/2018

Antigen Recognition(s):

Characterization Data [Compare Characterization Data]
  Western Blot

CPTC-EGFR-1 Western blot

CPTC-EGFR-1 antibody does not react with full length EGFR protein

Characterization SOP Files

  IPMS

CPTC-EGFR-1 iMRM

Immuno-MRM chromatogram of CPTC-EGFR-1 antibody with CPTC-EGFR peptide 4 (NCI ID#00115) as target

Background

Catalog Number:

CPTC-EGFR-2

Target Antigen:

Epidermal Growth Factor Receptor Peptide 4

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/15/2017

Antigen Recognition(s):

peptide

Background

Catalog Number:

CPTC-EGFR-3

Target Antigen:

Epidermal Growth Factor Receptor Peptide 4

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

02/15/2017

Antigen Recognition(s):

peptide

Background

NCI Identification Number:

00115

Antigen Name:

Epidermal Growth Factor Receptor Peptide 4

CPTC Name:

CPTC-EGFR

Keywords:

Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB; HER1; ERBB1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EC 2.7.10; NISBD2; PIG61; MENA

Function:

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

EGFR (Epidermal Growth Factor Receptor) is a Protein Coding gene. Diseases associated with EGFR include lung cancer and inflammatory skin and bowel disease, neonatal, 2. Among its related pathways are PI3K-Akt signaling pathway and PI-3K cascade. GO annotations related to this gene include identical protein binding and chromatin binding. An important paralog of this gene is TNK1.

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase of the ErbB family. Four members of the ErbB family have been identified; EGFR (ErbB1, HER1), ErbB2 (HER2), ErbB3 (HER3) and ErbB4 (HER4). EGFR signaling is initiated by ligand binding to the extracellular ligand binding domain. This initiates receptor homo-/hetero-dimerization and autophosphorylation by the intracellular kinase domain, resulting in receptor activation. Following activation, phosphorylation of cytoplasmic substrates occurs and a signaling cascade is initiated that drives many cellular responses, including changes in gene expression, cytoskeletal rearrangement, anti-apoptosis and increased cell proliferation.

Chromosomal Localization:

7p12

Expression System:

NCBI Accession Number:

NP_005219.2

Swiss Prot Accession Number:

P00533

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GSHQISLDNPD(pY)QQDFFPK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2090

Last Updated:

04/19/2018

Links

Characterization Data

SOPs

No SOPs available.

Don't have Adobe Reader™?

Get it for free at Adobe.com