Catalog Number:
CPTC-Calcyclin-1
RRID:
AB_1553421
Target Antigen:
Calcyclin (Prolactin Receptor Associated Protein)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:400
Human assay: U-2 OS fixed with PFA, dilution: 1:400
Human assay: U-251 MG fixed with PFA, dilution: 1:400
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-Calcylin is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.
Result: Positive
Western Blot using CPTC-Calcyclin-1 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as a positive control (lane 3).
Result: Positive
Western Blot using CPTC-Calcyclin-1 as primary Ab against cell lysate from SK-OV3 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-Calcyclin-2
Target Antigen:
Calcyclin (Prolactin Receptor Associated Protein)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
04/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Imaging mass cytometry on breast cancer tissue core using CPTC-Calcyclin-2 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (Bone Marrow, Spleen, Placenta, Prostate, Colon, Pancreas, Breast, Lung, Testis, Endometrium, Appendix, Kidney) and cancer tissues (Colon, Ovarian, Lung, Prostate).
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-S100A4-3 for NCI60 Cell Line Array at titer 1:250
0=NEGATIVE
1=WEAK
2=MODERATE
3=STRONG
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-Calcyclin-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot using CPTC-Calcyclin-2 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as positive control (lane 3).
Result: Positive
Western Blot using CPTC-Calcyclin-2 as primary Ab against cell lysate from SK-OV3 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-Calcyclin-3
RRID:
AB_10805145
Target Antigen:
Calcyclin (Prolactin Receptor Associated Protein)
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Human assay: U-251 MG fixed with PFA, dilution: 1:4.
Result: Positive
This antibody is not suitable for use in an Indirect ELISA format
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-Calcyclin-3 as primary Ab against Ag 10268 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG as a positive control (lane 3).
Result: Positive
Western Blot using CPTC-Calcyclin-3 as primary Ab against cell lysate from SK-OV3 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10268
Antigen Name:
Calcyclin (Prolactin Receptor Associated Protein)
CPTC Name:
CPTC-Calcyclin
Aliases:
Calcylin, S100A6; S100 calcium binding protein A6; 2A9; 5B10; CABP; CACY; OTTHUMP00000015472; OTTHUMP00000015473; PRA
Function:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
Chromosomal Localization:
1q21
Expression System:
E. Coli
Accession Number:
BC001431
UniProt Accession Number:
P06703
DNA Source:
HIP:HsCD00002783
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
4470
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQKELTIGSK
LQDAEIARLMEDLDRNKDQEVNFQEYVTFLGALALIYNEALKG
Native Sequence:
MACPLDQAIGLLVAIFHKYSGREGDKHTLSKKELKELIQKELTIGSKLQD
AEIARLMEDLDRNKDQEVNFQEYVTFLGALALIYNEALKG
Calculated Isoelectric Point:
5.32
Molecular Weight:
10452
Last Updated:
09/01/2008
PAGE of Calcyclin (rAg 10268) with molecular weight standards in lane 1.
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