Background
Catalog Number:
CPTC-CXCL9-2
RRID:
AB_2890080
Target Antigen:
C-X-C Motif Chemokine Ligand 9 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
External Links
- CPTAC Assay Portal CPTC-CXCL9-2
Purchase
- DSHB CPTC-CXCL9-2
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-CXCL9-2 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-CXCL9-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6238)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
NCI 60 Protein Array
CPTC-CXCL9-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CXCL9-2 Western Blot (Recombinant Protein)
Result: Positive
Western blot using CPTC-CXCL9-2 as primary antibody against chemokine (C-X-C motif) ligand 9 (CXCL9) human recombinant protein (lane 2). Expected molecular weight - 11.7 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CXCL9-1 Simple Western (tissue lysates)
Result: Negative
Automated WB (Simple Western) using CPTC-CXCL9-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 14 KDa. The antibody does not recognize the target in the tested tissue lysates.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CXCL9-2 Western Blot (Cell Lysate)
Result: Presumed Positive (with additional bands)
Western blot using CPTC-CXCL9-2 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 14.0 kDa. Cytochrome C was used as a loading control. HeLa is presumed positive. All other cell lines are negative.
Characterization SOP Files
Background
NCI Identification Number:
00362
Antigen Name:
C-X-C Motif Chemokine Ligand 9 Peptide 1
CPTC Name:
CPTC-CXCL9 Peptide 1
Aliases:
C-X-C Motif Chemokine Ligand 9; SCYB9; Monokine Induced By Gamma Interferon; Monokine Induced By Interferon-Gamma; Gamma-Interferon-Induced Monokine; Chemokine (C-X-C Motif) Ligand 9; Small-Inducible Cytokine B9; C-X-C Motif Chemokine 9; Crg-10; Humig; CMK; MIG; CXCL9; HuMIG
Function:
This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. CXCL9 (C-X-C Motif Chemokine Ligand 9) is a Protein Coding gene. Diseases associated with CXCL9 include Proliferative Glomerulonephritis and Paracoccidioidomycosis. Among its related pathways are Signaling by GPCR and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include cytokine activity and CXCR3 chemokine receptor binding. An important paralog of this gene is CXCL10.Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
Chromosomal Localization:
4q21.1
Accession Number:
NP_002407.1
UniProt Accession Number:
Q07325
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
IEIIATLK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
880
Last Updated:
09/02/2020
Links
- EnsEMBL - EnsEMBL CXCL9
- Entrez - Entrez CXCL9
- Uniprot - Uniprot Q07325
Characterization Data
SOPs
No SOPs available.
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