Carboxypeptidase E (mouse)


Background

Catalog Number:

CPTC-CPE-1

RRID:

AB_2756531

Target Antigen:

Carboxypeptidase E (mouse)

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2022

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (350.4 KB)

CPTC-CPE-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CPE-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot of CPTC-CPE-1 antibody with full length CPE recombinant protein (NCI ID#00236). Click image to enlarge

CPTC-CPE-1 Western blot

Result: Positive

Western blot of CPTC-CPE-1 antibody with full length CPE recombinant protein (NCI ID#00236).


Characterization SOP Files

Background

Catalog Number:

CPTC-CPE-2

RRID:

AB_2756532

Target Antigen:

Carboxypeptidase E (mouse)

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2022

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (328.9 KB)

CPTC-CPE-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CPE-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot of CPTC-CPE-2 antibody with full length CPE recombinant protein (NCI ID#00236). Click image to enlarge

CPTC-CPE-2 Western blot

Result: Positive

Western blot of CPTC-CPE-2 antibody with full length CPE recombinant protein (NCI ID#00236).


Characterization SOP Files

Background

Catalog Number:

CPTC-CPE-3

RRID:

AB_2756533

Target Antigen:

Carboxypeptidase E (mouse)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

01/03/2024

Antigen Recognition(s):

Recombinant Full-length

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (196.0 KB)

CPTC-CPE-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CPE-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot of CPTC-CPE-3 antibody with full length CPE recombinant protein (NCI ID#00236). Click image to enlarge

CPTC-CPE-3 Western blot

Result: Positive

Western blot of CPTC-CPE-3 antibody with full length CPE recombinant protein (NCI ID#00236).


Characterization SOP Files

Background

NCI Identification Number:

00236

Antigen Name:

Carboxypeptidase E (mouse)

CPTC Name:

CPTC-CPE

Aliases:

Carboxypeptidase E; Enkephalin Convertase; Carboxypeptidase H; Cobalt-Stimulated Chromaffin Granule Carboxypeptidase; Insulin Granule-Associated Carboxypeptidase; Prohormone-Processing Carboxypeptidase; EC 3.4.17.10; CPH; EC 3.4.17

Function:

This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.

CPE (Carboxypeptidase E) is a Protein Coding gene. Diseases associated with CPE include Hyperproinsulinemia and Phlebotomus Fever. Among its related pathways are Allograft rejection and Peptide hormone metabolism. GO annotations related to this gene include cell adhesion molecule binding and carboxypeptidase activity. An important paralog of this gene is CPD.

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

Chromosomal Localization:

4q32.3

Expression System:

Baculovirus

Accession Number:

NP_038522.2

UniProt Accession Number:

Q00493

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

Not provided

Extinction Coefficient:

Buffers:

PBS, pH 7.2

Expressed Sequence:

MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQQEDGIS
FEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVH
EPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTR
IHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYV
NEKEGGPNNHLLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGG
DLVANYPYDETRSGTAHEYSSCPDDAIFQSLARAYSSFNPVMSDPNRPPC
RKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPP
EETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVDGIDH
DVTSAKDGDYWRLLAPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELE
SFSERKEEEKEELMEWWKMMSETLNFHHHHHH

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

53000

Last Updated:

06/18/2020

Links

Characterization Data

SOPs

No SOPs available.

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