Background
Catalog Number:
CPTC-CPE-1
RRID:
AB_2756531
Target Antigen:
Carboxypeptidase E (mouse)
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2022
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-CPE-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (350.4 KB)
CPTC-CPE-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CPE-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CPE-1 Western blot
Result: Positive
Western blot of CPTC-CPE-1 antibody with full length CPE recombinant protein (NCI ID#00236).
Characterization SOP Files
Background
Catalog Number:
CPTC-CPE-2
RRID:
AB_2756532
Target Antigen:
Carboxypeptidase E (mouse)
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
08/24/2022
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-CPE-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (328.9 KB)
CPTC-CPE-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CPE-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CPE-2 Western blot
Result: Positive
Western blot of CPTC-CPE-2 antibody with full length CPE recombinant protein (NCI ID#00236).
Characterization SOP Files
Background
Catalog Number:
CPTC-CPE-3
RRID:
AB_2756533
Target Antigen:
Carboxypeptidase E (mouse)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
01/03/2024
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-CPE-3
External Links
- Human Protein Atlas CPTC-CPE-3
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (196.0 KB)
CPTC-CPE-3 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CPE-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CPE-3 Western blot
Result: Positive
Western blot of CPTC-CPE-3 antibody with full length CPE recombinant protein (NCI ID#00236).
Characterization SOP Files
Background
NCI Identification Number:
00236
Antigen Name:
Carboxypeptidase E (mouse)
CPTC Name:
CPTC-CPE
Aliases:
Carboxypeptidase E; Enkephalin Convertase; Carboxypeptidase H; Cobalt-Stimulated Chromaffin Granule Carboxypeptidase; Insulin Granule-Associated Carboxypeptidase; Prohormone-Processing Carboxypeptidase; EC 3.4.17.10; CPH; EC 3.4.17
Function:
This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.
CPE (Carboxypeptidase E) is a Protein Coding gene. Diseases associated with CPE include Hyperproinsulinemia and Phlebotomus Fever. Among its related pathways are Allograft rejection and Peptide hormone metabolism. GO annotations related to this gene include cell adhesion molecule binding and carboxypeptidase activity. An important paralog of this gene is CPD.
Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.
Chromosomal Localization:
4q32.3
Expression System:
Baculovirus
Accession Number:
NP_038522.2
UniProt Accession Number:
Q00493
DNA Source:
N/A
Immunogen:
Recombinant Full Length Protein
Vector Name:
Not provided
Extinction Coefficient:
Buffers:
PBS, pH 7.2
Expressed Sequence:
MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQQEDGIS
FEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVH
EPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTR
IHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYV
NEKEGGPNNHLLKNLKKIVDQNSKLAPETKAVIHWIMDIPFVLSANLHGG
DLVANYPYDETRSGTAHEYSSCPDDAIFQSLARAYSSFNPVMSDPNRPPC
RKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPP
EETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVDGIDH
DVTSAKDGDYWRLLAPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELE
SFSERKEEEKEELMEWWKMMSETLNFHHHHHH
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
53000
Last Updated:
06/18/2020
Links
- EnsEMBL - EnsEMBL CPE
- Entrez - Entrez CPE
- Uniprot - Uniprot Q00493
Characterization Data
SOPs
No SOPs available.
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