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Chloride Intracellular Channel 1

Antibody: CPTC-CLIC1-1
Background

Catalog Number:

CPTC-CLIC1-1

RRID:

AB_1553432

Target Antigen:

Chloride Intracellular Channel 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (138.2 KB)

CPTC-CLIC1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immuno-histochemistry of CPTC-CLIC1-1 for NCI60 Cell Line Array at titer 1:250 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) Click image to enlarge

CPTC-CLIC1-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-CLIC1-1 for NCI60 Cell Line Array at titer 1:250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-CLIC1-1. Titer: 1:250 Click image to enlarge

CPTC-CLIC1-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-CLIC1-1. Titer: 1:250


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Human assay: A-431 fixed with PFA, dilution: 1:200 Human assay: U-2 OS fixed with PFA, dilution: 1:200 Human assay: U-251 MG fixed with PFA, dilution: 1:200 Click image to enlarge

CPTC-CLIC1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-CLIC1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CLIC1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-CLIC1-1 NCI60 Protein Array

Result: Positive

Protein Array in which CPTC-CLIC1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250. Click image to enlarge

CPTC-CLIC1-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CLIC1-1 as primary Ab against Ag 10458 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-CLIC1-1 Western Blot

Result: Positive

Western Blot using CPTC-CLIC1-1 as primary Ab against Ag 10458 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-CLIC1-2
Background

Catalog Number:

CPTC-CLIC1-2

RRID:

AB_2291933

Target Antigen:

Chloride Intracellular Channel 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

08/24/2021

Antigen Recognition(s):

Recombinant Full-length

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (133.6 KB)

CPTC-CLIC1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Click to enlarge image Immunohistochemistry of CPTC-CLIC1-2 for NCI60 Cell Line Array. Data scored as: 0=NEGATIVE 1=WEAK (red) 2=MODERATE (blue) 3=STRONG (green) Titer: 1:250 Click image to enlarge

CPTC-CLIC1-2 IHC NCI60

Result: Positive

Immunohistochemistry of CPTC-CLIC1-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
Titer: 1:250


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-CLIC1-2. Titer: 1:250 Click image to enlarge

CPTC-CLIC1-2 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of ovarian cancer showing cytoplasmic staining using Antibody CPTC-CLIC1-2. Titer: 1:250


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-CLIC1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CLIC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-CLIC1-2 Protein Array

Result: Positive

Protein Array in which CPTC-CLIC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CLIC1-2 as primary Ab against Ag 10458 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-CLIC1-2 Western Blot

Result: Positive

Western Blot using CPTC-CLIC1-2 as primary Ab against Ag 10458 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Chloride Intracellular Channel 1
Background

NCI Identification Number:

10458

Antigen Name:

Chloride Intracellular Channel 1

CPTC Name:

CPTC-CLIC1

Aliases:

CLIC1; G6; NCC27; OTTHUMP00000029131; OTTHUMP00000029133; OTTHUMP00000029137; OTTHUMP00000174486; hRNCC; p64CLCP

Function:

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes
including stabilization of cell membrane potential,transepithelial transport, maintenance of
intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity.

Chromosomal Localization:

6p22.1 - p21.2

Expression System:

E. Coli

Accession Number:

NM_001288

UniProt Accession Number:

O00299

DNA Source:

HIP:HsCD00001712

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

17608

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVD
TKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLA
ALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSP
LPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGF
TIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK

Native Sequence:

MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKR
RTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALN
PESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPE
EVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIP
EAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK

Calculated Isoelectric Point:

5.09

Molecular Weight:

27195

Last Updated:

09/01/2008

Links

Characterization Data

Gel

Click to enlarge image PAGE of CLIC1 (rAg 10458) with molecular weight standards in lane 1.
Click image to enlarge

CPTC-CLIC1 (rAg 10458) PAGE

PAGE of CLIC1 (rAg 10458) with molecular weight standards in lane 1.

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SOPs

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