Background
Catalog Number:
CPTC-CHKA-1
Target Antigen:
Choline Kinase Alpha
Isotype:
IgG1
Species:
Mouse Recombinant Cloned Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-CHKA-1
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-CHKA-1 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CHKA-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-CHKA-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CHKA-1 Western blot
Result: Positive
Western Blot using CPTC-CHKA-1 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Background
Catalog Number:
CPTC-CHKA-2
RRID:
AB_2617233
Target Antigen:
Choline Kinase Alpha
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-CHKA-2
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-CHKA-2 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CHKA-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-CHKA-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CHKA-2 Western blot
Result: Positive
Western Blot using CPTC-CHKA-2 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-CHKA-3
RRID:
AB_2617234
Target Antigen:
Choline Kinase Alpha
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-CHKA-3
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-CHKA-3 Evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CHKA-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-CHKA-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CHKA-3 Western blot
Result: Positive
Western Blot using CPTC-CHKA-3 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00008
Antigen Name:
Choline Kinase Alpha
CPTC Name:
CPTC-CHKA
Aliases:
choline kinase alpha1; Ck; EK; CHK; CK1; choline kinase; Ethanolamine kinase; EC 2.7.1.32; CHETK-alpha; EC 2.7.1.82
Function:
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded
by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes
the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene.
Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and
ethanolamine. Has higher activity with choline.
Chromosomal Localization:
11q13.2
Expression System:
E. coli
Accession Number:
NM_001277
UniProt Accession Number:
P35790
DNA Source:
Mammalian Gene Collection
Immunogen:
Recombinant Domain
Vector Name:
pET28-LIC
Extinction Coefficient:
Buffers:
Expressed Sequence:
MGSSHHHHHHSSGLVPRGSpqppadeqpeprtrrraylwckeflpgawrg
lredefhisvirgglsnmlfqcslpdttatlgdeprkvllrlygailqmr
scnkegseqaqkenefqgaeamvlesvmfailaerslgpklygifpqgrl
eqfipsrrldteelslpdisaeiaekmatfhgmkmpfnkepkwlfgtmek
ylkevlrikfteesrikklhkllsynlplelenlrsllestpspvvfchn
dcqegnilllegrensekqklmlidfeyssynyrgfdignhfcewmydys
yekypffranirkyptkkqqlhfissylpafqndfenlsteeksiikeem
llevnrfalashflwglwsivqakissiefgymdyaqarfdayfhqkrkl
gv
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
46923
Last Updated:
11/20/2012
Links
- EnsEMBL - EnsEMBL CHKA
- Entrez - Entrez CHKA
- Uniprot - Uniprot P35790
Characterization Data
Gel
Click image to enlarge
CPTC-CHKA PAGE
PAGE of CHKA (Ag 0008) with molecular weight standards in lane 1
Characterization SOP Files
SOPs
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