Choline Kinase Alpha


Background

Catalog Number:

CPTC-CHKA-1

Target Antigen:

Choline Kinase Alpha

Isotype:

IgG1

Species:

Mouse Recombinant Cloned Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-CHKA-1 Evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHKA-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CHKA-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CHKA-1 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-CHKA-1 Western blot

Result: Positive

Western Blot using CPTC-CHKA-1 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

Background

Catalog Number:

CPTC-CHKA-2

RRID:

AB_2617233

Target Antigen:

Choline Kinase Alpha

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-CHKA-2 Evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHKA-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CHKA-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CHKA-2 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-CHKA-2 Western blot

Result: Positive

Western Blot using CPTC-CHKA-2 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

Background

Catalog Number:

CPTC-CHKA-3

RRID:

AB_2617234

Target Antigen:

Choline Kinase Alpha

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-CHKA-3 Evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHKA-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CHKA-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CHKA-3 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-CHKA-3 Western blot

Result: Positive

Western Blot using CPTC-CHKA-3 as primary Ab against CHKA (rAg 00008) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

Background

NCI Identification Number:

00008

Antigen Name:

Choline Kinase Alpha

CPTC Name:

CPTC-CHKA

Aliases:

choline kinase alpha1; Ck; EK; CHK; CK1; choline kinase; Ethanolamine kinase; EC 2.7.1.32; CHETK-alpha; EC 2.7.1.82

Function:

The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded
by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes
the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene.

Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and
ethanolamine. Has higher activity with choline.

Chromosomal Localization:

11q13.2

Expression System:

E. coli

Accession Number:

NM_001277

UniProt Accession Number:

P35790

DNA Source:

Mammalian Gene Collection

Immunogen:

Recombinant Domain

Vector Name:

pET28-LIC

Extinction Coefficient:

Buffers:

Expressed Sequence:

MGSSHHHHHHSSGLVPRGSpqppadeqpeprtrrraylwckeflpgawrg
lredefhisvirgglsnmlfqcslpdttatlgdeprkvllrlygailqmr
scnkegseqaqkenefqgaeamvlesvmfailaerslgpklygifpqgrl
eqfipsrrldteelslpdisaeiaekmatfhgmkmpfnkepkwlfgtmek
ylkevlrikfteesrikklhkllsynlplelenlrsllestpspvvfchn
dcqegnilllegrensekqklmlidfeyssynyrgfdignhfcewmydys
yekypffranirkyptkkqqlhfissylpafqndfenlsteeksiikeem
llevnrfalashflwglwsivqakissiefgymdyaqarfdayfhqkrkl
gv

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

46923

Last Updated:

11/20/2012

Links

Characterization Data

Gel

Click to enlarge image PAGE of CHKA (Ag 0008) with molecular weight standards in lane 1
Click image to enlarge

CPTC-CHKA PAGE

PAGE of CHKA (Ag 0008) with molecular weight standards in lane 1

SOPs

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