Checkpoint Kinase 1 peptide 2


Background

Catalog Number:

CPTC-CHEK1-4

Target Antigen:

Checkpoint Kinase 1 peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image mmuno-MRM chromatogram of CPTC-CHEK1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3282) Click image to enlarge

CPTC-CHEK1-4 iMRM

mmuno-MRM chromatogram of CPTC-CHEK1-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3282)

Background

Catalog Number:

CPTC-CHEK1-5

Target Antigen:

Checkpoint Kinase 1 peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image mmuno-MRM chromatogram of CPTC-CHEK1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3223) Click image to enlarge

CPTC-CHEK1-5 iMRM

mmuno-MRM chromatogram of CPTC-CHEK1-5 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3223)

Background

NCI Identification Number:

00206

Antigen Name:

Checkpoint Kinase 1 peptide 2

CPTC Name:

CPTC-CHEK1 peptide 2

Keywords:

CHK1; Checkpoint Kinase 1; Cell Cycle Checkpoint Kinase; Checkpoint Kinase-1; CHK1 Checkpoint Homolog; CHK1 (Checkpoint, S.Pombe) Homolog; Chk1-S; Checkpoint, S. Pombe, Homolog Of; Serine/Threonine-Protein Kinase Chk1

Function:

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint
mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to
integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

CHEK1 (checkpoint kinase 1) is a protein-coding gene. Diseases associated with CHEK1 include hypoxia. GO annotations related to this gene include histone kinase activity (H3-T11 specific) and identical protein binding.

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.

Isoform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.

Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. Two subtypes have so far been identified, Chk1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates Chk1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Therefore Cdc25 cannot remove the inhibitory phosphorylation on mitotic promoting factor (MPF) and entry into mitosis is prohibited. In addition, Chks have a role in the physiological stress of hypoxia/reoxygenation.




Chromosomal Localization:

11q24.2

Expression System:

NCBI Accession Number:

NP_001107593.1

Swiss Prot Accession Number:

O14757

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

YSS(pS)QPEPR (pS317)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

03/08/2018

Links

Characterization Data

SOPs

No SOPs available.

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