Checkpoint Kinase 1 peptide 1


Background

Catalog Number:

CPTC-CHEK1-1

Target Antigen:

Checkpoint Kinase 1 peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

10/15/2019

Antigen Recognition(s):

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

SOP:

Characterization Data [Compare Characterization Data]
  IPMS

CPTC-CHEK1-1 IPMS (phosporylated peptide)

Result: Positive

CPTC-CHEK1-1 Antibody did not react with phoshorylated CPTC-CHEK1 peptide 1 (NCI 00093) as the target antigen using Immuno-Precipitation Mass Spectrometry


  IPMS
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-CHEK1-1 antibody with CPTC-CHEK1 peptide 1 (NCI 00093; non-phosphorylated) as the target antigen. Click image to enlarge

CHEK1-1 IPMS (phosphorylated peptide)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-CHEK1-1 antibody with CPTC-CHEK1 peptide 1 (NCI 00093; non-phosphorylated) as the target antigen.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHEK1-1 pS peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHEK1-1 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Background

Catalog Number:

CPTC-CHEK1-2

Target Antigen:

Checkpoint Kinase 1 peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/06/2019

Antigen Recognition(s):

Peptide

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

SOP:

Characterization Data [Compare Characterization Data]
  IPMS
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-CHEK1-2 antibody with CPTC-CHEK1 peptide 1 (NCI 00093; phosphorylated) as the target antigen. Click image to enlarge

CPTC-CHEK1-2 IPMS (phosporylated peptide)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-CHEK1-2 antibody with CPTC-CHEK1 peptide 1 (NCI 00093; phosphorylated) as the target antigen.


  IPMS

CPTC-CHEK1-2 IPMS (non-phosphorylated peptide)

Result: Positive

CPTC-CHEK1-2 Antibody did not react with non-phoshorylated CPTC-CHEK1 peptide 1 (NCI 00093) as the target antigen using Immuno-Precipitation Mass Spectrometry


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHEK1-2 pS peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHEK1-2 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Background

Catalog Number:

CPTC-CHEK1-3

RRID:

AB_2722046

Target Antigen:

Checkpoint Kinase 1 peptide 1

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

11/13/2019

Antigen Recognition(s):

Peptide

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IPMS
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-CHEK1-3 antibody with CPTC-CHEK1 peptide 1 (NCI 00093; phosphorylated) as the target antigen. Click image to enlarge

CPTC-CHEK1-3 IPMS (phosporylated peptide)

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-CHEK1-3 antibody with CPTC-CHEK1 peptide 1 (NCI 00093; phosphorylated) as the target antigen.


  IPMS

CPTC-CHEK1-3 IPMS (non-phosphorylated peptide)

Result: Positive

CPTC-CHEK1-3 Antibody did not react with non-phoshorylated CPTC-CHEK1 peptide 1 (NCI 00093) as the target antigen using Immuno-Precipitation Mass Spectrometry


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CHEK1-3 pS peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated phospho-peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA

CPTC-CHEK1-3 peptide ELISA

Result: Negative

CPTC-CHEK1-3 did not react with the non-phosphorylated form of the target peptide in the Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate).


Background

NCI Identification Number:

00093

Antigen Name:

Checkpoint Kinase 1 peptide 1

CPTC Name:

CPTC-CHEK1 peptide 1

Keywords:

CHK1; Checkpoint Kinase 1; Serine/Threonine-Protein Kinase Chk1; Cell Cycle Checkpoint Kinase; CHK1 Checkpoint Homolog; EC 2.7.11.1; CHK1; Checkpoint, S. Pombe, Homolog Of, 1; CHK1 (Checkpoint, S.Pombe) Homolog; CHK1 Checkpoint Homolog (S. Pombe); Checkpoint Kinase-1; EC 2.7.11; Chk1-S

Function:

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. CHEK1 (Checkpoint Kinase 1) is a Protein Coding gene. Diseases associated with CHEK1 include Ataxia-Telangiectasia and Breast Cancer. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Human cytomegalovirus infection. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell-cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest. soform 2: Endogenous repressor of isoform 1, interacts with, and antagonizes CHK1 to promote the S to G2/M phase transition.Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. Two subtypes have so far been identified, Chk1 and Chk2. They are essential components to delay cell cycle progression in all cells and act at all three cell cycle checkpoints.

Chromosomal Localization:

11q24.2

NCBI Accession Number:

NP_001107593.1

UniProt Accession Number:

O14757

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CKYSS(pS)QPEPRT

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

10/23/2019

Links

Characterization Data

SOPs

No SOPs available.

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