CD44 molecule (Indian blood group) Peptide 1


Background

Catalog Number:

CPTC-CD44-1

Target Antigen:

CD44 molecule (Indian blood group) Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/25/2020

Antigen Recognition(s):

Peptide, Recombinant Full-length

SOP:

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (404.8 KB)

CPTC-CD44-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CD44-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CD44-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CD44-1 as primary Ab against HEK293T cell lysate containing CD44 (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CD44-1 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-CD44-1 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-CD44-1 as primary Ab against HEK293T cell lysate containing CD44 (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the CD44-1 Ab as the IgG control (lane 3).


Background

NCI Identification Number:

00018

Antigen Name:

CD44 molecule (Indian blood group) Peptide 1

CPTC Name:

CPTC-CD44 Peptide 1

Keywords:

CD44 Antigen; GP90 Lymphocyte Homing/Adhesion Receptor; Hermes Antigen; CD44R; CD44 Antigen (Homing Function And Indian Blood Group System); CDW44; Cell Surface Glycoprotein CD44; Chondroitin Sulfate Proteoglycan 8; Epican; Hematopoietic Cell E- And L-Selectin Ligand; Homing Function And Indian Blood Group System; CDw44; PGP-1; Phagocytic Glycoprotein I; MDU2; MDU3; MIC4; CSPG8; HCELL; MC56; IN; Extracellular Matrix Receptor III; Heparan Sulfate Proteoglycan; Hyaluronate Receptor; ECMR-III; HUTCH-I; LHR; Soluble CD44; CDW44; CD44

Function:

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. CD44 (CD44 Molecule (Indian Blood Group)) is a Protein Coding gene. Diseases associated with CD44 include Superficial Keratitis and Intraductal Papilloma. Among its related pathways are Degradation of the extracellular matrix and Cell surface interactions at the vascular wall. Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and cytokine receptor activity. An important paralog of this gene is LYVE1.Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Chromosomal Localization:

11p13

NCBI Accession Number:

NP_000601.3

UniProt Accession Number:

P16070

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TFIPVTSAK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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