Background
Catalog Number:
CPTC-CA8-1
RRID:
AB_10804670
Target Antigen:
Carbonic Anhydrase VIII
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-CA8-1
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-CA8-1 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on ovarian cancer tissue core using CPTC-CA8-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (colon, pancreas, breast, lung, testis, endometrium, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (93.2 KB)
CPTC-CA8-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-CA8-1 IHC NCI60
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-CA8-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Indirect ELISA
CPTC-CA8-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-CA8-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CA8-1 Western blot
Result: Positive
Western Blot using CPTC-CA8-1 as primary Ab against Ag 10903 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-CA8-2
RRID:
AB_10805875
Target Antigen:
Carbonic Anhydrase VIII
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length
SOP:
Purchase
- DSHB CPTC-CA8-2
External Links
- Human Protein Atlas CPTC-CA8-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (110.6 KB)
CPTC-CA8-2 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-CA8-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-CA8-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CA8-2 Western blot
Result: Positive
Western Blot using CPTC-CA8-2 as primary Ab against Ag 10903 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10903
Antigen Name:
Carbonic Anhydrase VIII
CPTC Name:
CPTC-CA8
Aliases:
CA-VIII; CALS; CARP; MGC120502; MGC99509
Function:
The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form.
Does not have a carbonic anhydrase catalytic activity.
Chromosomal Localization:
8q11 - q12
Expression System:
E. Coli
Accession Number:
BC108929.2
UniProt Accession Number:
P35219
DNA Source:
OB : MHS4426-99622804
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
46535
Buffers:
PBS
Expressed Sequence:
SNAMADLSFIEDTVAFPEKEEDEEEEEEGVEWGYEEGVEWGLVFPDANGE
YQSPINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKS
VLSGGPLPQGHEFELYEVRFHWGRENQRGSEHTVNFKAFPMELHLIHWNS
TLFGSIDEAVGKPHGIAIIALFVQIGKEHVGLKAVTEILQDIQYKGKSKT
IPCFNPNTLLPDPLLRDYWVYEGSLTIPPCSEGVTWILFRYPLTISQLQI
EEFRRLRTHVKGAELVEGCDGILGDNFRPTQPLSDRVIRAAFQ
Native Sequence:
MADLSFIEDTVAFPEKEEDEEEEEEGVEWGYEEGVEWGLVFPDANGEYQS
PINLNSREARYDPSLLDVRLSPNYVVCRDCEVTNDGHTIQVILKSKSVLS
GGPLPQGHEFELYEVRFHWGRENQRGSEHTVNFKAFPMELHLIHWNSTLF
GSIDEAVGKPHGIAIIALFVQIGKEHVGLKAVTEILQDIQYKGKSKTIPC
FNPNTLLPDPLLRDYWVYEGSLTIPPCSEGVTWILFRYPLTISQLQIEEF
RRLRTHVKGAELVEGCDGILGDNFRPTQPLSDRVIRAAFQ
Calculated Isoelectric Point:
4.78
Molecular Weight:
32.97
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL CA8
- Entrez - Entrez CA8
- Uniprot - Uniprot P35219
Characterization Data
Gel
Click image to enlarge
CPTC-CA8 PAGE
PAGE of Ag 10903(with molecular weight standards in lane 1)
Characterization SOP Files
SOPs
Don't have Adobe Reader™?
Get it for free at Adobe.com