BRCA2, DNA Repair Associated Peptide 1


Background

Catalog Number:

CPTC-BRCA2-1

Target Antigen:

BRCA2, DNA Repair Associated Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/26/2018

Antigen Recognition(s):

peptide, phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-BRCA2-1 antibody (see CPTAC assay portal for details:  https://assays.cancer.gov/CPTAC-3280) Click image to enlarge

CPTC-BRCA2-1

Immuno-MRM chromatogram of CPTC-BRCA2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3280)

Background

NCI Identification Number:

00194

Antigen Name:

BRCA2, DNA Repair Associated Peptide 1

CPTC Name:

CPTC-BRCA2 Peptide 1

Keywords:

BRCA2, DNA Repair Associated; BRCA1/BRCA2-Containing Complex, Subunit 2; Fanconi Anemia Group D1 Protein; Breast Cancer 2, Early Onset; FANCD1; FACD; Breast And Ovarian Cancer Susceptibility Gene, Early Onset; Breast And Ovarian Cancer Susceptibility Protein 2; Breast Cancer Type 2 Susceptibility Protein; Fanconi Anemia, Complementation Group D1; Breast Cancer 2 Tumor Suppressor; Breast Cancer 2; Mutant BRCA2; BROVCA2; XRCC11; BRCC2; PNCA2; FANCD; FAD1; GLM3; FAD

Function:

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

RCA2 (BRCA2, DNA Repair Associated) is a Protein Coding gene. Diseases associated with BRCA2 include Fanconi Anemia, Complementation Group D1 and Breast Cancer. Among its related pathways are Pathways in cancer and Meiosis. GO annotations related to this gene include protease binding and histone acetyltransferase activity.

nvolved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA). Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51-ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SHFM1/DSS1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination

Chromosomal Localization:

13q13.1

Expression System:

NCBI Accession Number:

NP_000050.1

Swiss Prot Accession Number:

P51587

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

T(pS)VSQTSLLEAK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

02/16/2017

Links

Characterization Data

SOPs

No SOPs available.

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