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AXL Receptor Tyrosine Kinase Peptide 1

Antibody: CPTC-AXL-1
Background

Catalog Number:

CPTC-AXL-1

Target Antigen:

AXL Receptor Tyrosine Kinase Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (324.7 KB)

CPTC-AXL-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM screening of antibody CPTC-AXL-1 against synthetic peptide KIYNGDY(pY)RQGRIAK (AXL Receptor Tyrosine Kinase Peptide 1) and its correspondent non-phosphorylated peptide (KIYNGDYYRQGRIAK). The antibody was able to pull down only the phosphorylated peptide. Click image to enlarge

CPTC-AXL-1 iMRM (synthetic peptide)

Result: Positive

Immuno-MRM screening of antibody CPTC-AXL-1 against synthetic peptide KIYNGDY(pY)RQGRIAK (AXL Receptor Tyrosine Kinase Peptide 1) and its correspondent non-phosphorylated peptide (KIYNGDYYRQGRIAK). The antibody was able to pull down only the phosphorylated peptide.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot usign CPTC-AXL-1 as primary antibody against cell lysates of MDA-MB-231 cells treated (lane 2) and not treated (lane 3) with GAS6 (400 ng/mL0 for 10 minutes, after overnight starvation). Molecular weight standards are also included (lane 1). The antibody was able to detect the phosphorylated target protein in the GAS6 treated cell lysate. Expected molecultar weight for AXL is about 98 KDa. Click image to enlarge

CPTC-AXL-1 western blot with lysates

Result: Presumed Positive (with additional bands)

Western Blot usign CPTC-AXL-1 as primary antibody against cell lysates of MDA-MB-231 cells treated (lane 2) and not treated (lane 3) with GAS6 (400 ng/mL0 for 10 minutes, after overnight starvation). Molecular weight standards are also included (lane 1). The antibody was able to detect the phosphorylated target protein in the GAS6 treated cell lysate. Expected molecultar weight for AXL is about 98 KDa.


Characterization SOP Files

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Antigen: AXL Receptor Tyrosine Kinase Peptide 1
Background

NCI Identification Number:

00339

Antigen Name:

AXL Receptor Tyrosine Kinase Peptide 1

CPTC Name:

CPTC-AXL Peptide 1

Aliases:

AXL Receptor Tyrosine Kinase; UFO; Tyrosine-Protein Kinase Receptor UFO; AXL Oncogene; EC 2.7.10.1; JTK11; Tyro7; ARK; AXL Transforming Sequence/Gene; EC 2.7.10; AXL

Function:

The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.AXL (AXL Receptor Tyrosine Kinase) is a Protein Coding gene. Diseases associated with AXL include Hypogonadotropic Hypogonadism 7 With Or Without Anosmia and Lymphocytic Choriomeningitis. Among its related pathways are Signaling by GPCR and Activation of cAMP-Dependent PKA. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MERTK.Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

Chromosomal Localization:

19q13.2

Accession Number:

NP_068713.2

UniProt Accession Number:

P30530

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

KIYNGDY(pY)RQGRIAK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

09/14/2020

Links

Characterization Data

SOPs

No SOPs available.

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