Background
Catalog Number:
CPTC-ARAF-1
RRID:
AB_2722020
Target Antigen:
A-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/22/2022
Antigen Recognition(s):
Peptide
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-ARAF-1
Publications
- A dataset describing a suite of novel antibody reagents for the RAS signaling network (PMID: 31467290)
- Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling (PMID: 34671754)
Characterization Data [Compare Characterization Data]
Immuno-MRM
CPTC-ARAF-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-ARAF-1 antibody with CPTC-ARAF peptide 1 (NCI ID#145) as target
Background
NCI Identification Number:
00145
Antigen Name:
A-Raf Proto-Oncogene, Serine/Threonine Kinase Peptide 1
CPTC Name:
CPTC-ARAF Peptide 1
Aliases:
A-Raf Proto-Oncogene, Serine/Threonine Kinase; V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog 1; Proto-Oncogene A-Raf-1; Proto-Oncogene Pks; EC 2.7.11.1; ARAF1; PKS2; Ras-Binding Protein DA-Raf; Proto-Oncogene A-Raf; Oncogene ARAF1; EC 2.7.11; A-RAF; RAFA1; PKS
Function:
This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ARAF (A-Raf Proto-Oncogene, Serine/Threonine Kinase) is a Protein Coding gene. Diseases associated with ARAF include pallister-killian syndrome and mycobacterium marinum. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Signaling by GPCR. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is RAF1.
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade.
Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Raf kinases, a family of three serine/threonine kinases, are part of the Ras-MAPK signaling cascade and phosphorylate MEK. Upon growth factor stimulation, Raf-1 (c-Raf) is activated by GTP-bound Ras and recruited to the cell membrane. This activation process is tightly regulated.
Chromosomal Localization:
Xp11.3
Accession Number:
NP_001243125.1
UniProt Accession Number:
P10398
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GLNQDCCVVYR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1210
Last Updated:
07/12/2016
Links
- Entrez - Entrez ARAF
- EnsEMBL - EnsEMBL ARAF
- Uniprot - Uniprot P10398
Characterization Data
SOPs
No SOPs available.
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