Annexin A4 Peptide 1


Background

Catalog Number:

CPTC-ANXA4-1

Target Antigen:

Annexin A4 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/10/2019

Antigen Recognition(s):

Peptide, Recombinant Full-length, Native Protein

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-ANXA4-1 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-ANXA4-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-ANXA4-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-ANXA4-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-ANXA4-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-ANXA4-1 as primary Ab against full-length recombinant Ag 10323 (lane 2). Also included are molecular wt. standards (lane 1) and the SAIC-14C Ab as positive control (lane 3). Click image to enlarge

CPTC-ANXA4-1 Western blot

Result: Positive

Western Blot using CPTC-ANXA4-1 as primary Ab against full-length recombinant Ag 10323 (lane 2). Also included are molecular wt. standards (lane 1) and the SAIC-14C Ab as positive control (lane 3).


Background

NCI Identification Number:

00024

Antigen Name:

Annexin A4 Peptide 1

CPTC Name:

CPTC-ANXA4 Peptide 1

Keywords:

ANXA4; ANX4; Annexin-4; Chromobindin-4; DKFZp686H02120; MGC75105; P32.5; P33/41; PAP-II; PIG28; PP4-X; ZAP36

Function:

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis (By similarity).

Chromosomal Localization:

2p13.3

NCBI Accession Number:

NP_001144.1

Swiss Prot Accession Number:

P09525

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DEGNYLDDALVR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

11/06/2019

Links

Characterization Data

SOPs

No SOPs available.

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