Catalog Number:
CPTC-GLO1-1
RRID:
AB_1553719
Target Antigen:
Lactoylglutathione Lyase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Kinetic titration data for GLO1-1 Ab (615A3.1) using Biacore SPR method
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-GLO1-1 for NCI60 Cell Line Array at titer Titer: 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:50
Human assay: U-2 OS fixed with PFA, dilution: 1:50
Human assay: U-251 MG fixed with PFA, dilution: 1:50
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-GLO1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.
Result: Positive
Western Blot using CPTC-GLO1-1 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Result: Positive
Western Blot using CPTC-GLO1-1 as primary Ab against cell lysate from NCI-H522 cells (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GLO1-2
RRID:
AB_1553721
Target Antigen:
Lactoylglutathione Lyase
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Result: Positive
Immunohistochemistry of CPTC-GLO1-2 for NCI60 Cell Line Array. Data scored as:
0=NEGATIVE
1=WEAK (red)
2=MODERATE (blue)
3=STRONG (green)
TITER: 1:2500
Result: Positive
Tissue Micro-Array(TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-GLO1-2. Titer: 1:2500
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-GLO1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot using CPTC-GLO1-2 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GLO1-3
RRID:
AB_2109905
Target Antigen:
Lactoylglutathione Lyase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
Kinetic titration data for GLO1-3 Ab (615A28.1) using Biacore SPR method
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-GLO1-3 for NCI60 Cell Line Array at titer 1:100
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-AKR1B1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.
Result: Positive
Western Blot using CPTC-GLO1-3 as primary Ab against GLO1 (Ag 10246) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10246
Antigen Name:
Lactoylglutathione Lyase
CPTC Name:
CPTC-GLO1
Aliases:
GLO1; glyoxalase 1; Aldoketomutase; EC 4.4.1.5; GLOD1; GLY1; Methylglyoxalase; GLX1; ketone-aldehyde mutase; S-D-lactoyl glutathione methyl glyoxal lyase; OTTHUMP00000016339
Function:
The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere.
Chromosomal Localization:
6p21.3 p21.1
Expression System:
E. Coli
Accession Number:
NM_006708
UniProt Accession Number:
Q04760
DNA Source:
HIP:HsCD00000982
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
25565
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLD
FYTRVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKA
TLELTHNWGTEDDATQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGV
KFVKKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Native Sequence:
MAEPQPPSGGLTDEAALSCCSDADPSTKDFLLQQTMLRVKDPKKSLDFYT
RVLGMTLIQKCDFPIMKFSLYFLAYEDKNDIPKEKDEKIAWALSRKATLE
LTHNWGTEDDATQSYHNGNSDPRGFGHIGIAVPDVYSACKRFEELGVKFV
KKPDDGKMKGLAFIQDPDGYWIEILNPNKMATLM
Calculated Isoelectric Point:
5.24
Molecular Weight:
20992
Last Updated:
08/22/2020
SDS-PAGE of CPTC-GLO1 with molecular weight markers (in Lane 1)
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