C-X-C Motif Chemokine Ligand 9


Background

Catalog Number:

CPTC-CXCL9-1

RRID:

AB_2617243

Target Antigen:

C-X-C Motif Chemokine Ligand 9

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

08/25/2021

Antigen Recognition(s):

Recombinant Full-length

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Characterization Data [Compare Characterization Data]
  IHC HPA

CPTC-CXCL9-1 Evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC NCI60

CPTC-CXCL9-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  IHC Tissue

CPTC-CXCL9-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-CXCL9-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CXCL9-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-CXCL9-1 as primary Ab against CXCL9 (rAg 11056) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-CXCL9-1 Western blot

Result: Positive

Western Blot using CPTC-CXCL9-1 as primary Ab against CXCL9 (rAg 11056) in lane 2. Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

NCI Identification Number:

11056

Antigen Name:

C-X-C Motif Chemokine Ligand 9

CPTC Name:

CPTC-CXCL9

Aliases:

chemokine (C-X-C motif) ligand 9; C-X-C Motif Chemokine Ligand 9; SCYB9; Monokine Induced By Gamma Interferon; Monokine Induced By Interferon-Gamma; Gamma-Interferon-Induced Monokine; Chemokine (C-X-C Motif) Ligand 9; Small-Inducible Cytokine B9; C-X-C Motif Chemokine 9; Crg-10; CMK; MIG; CXCL9; HuMIG

Function:

This antimicrobial gene encodes a protein thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils. CXCL9 (C-X-C Motif Chemokine Ligand 9) is a Protein Coding gene. Diseases associated with CXCL9 include Proliferative Glomerulonephritis and Paracoccidioidomycosis. Among its related pathways are Signaling by GPCR and PEDF Induced Signaling. Gene Ontology (GO) annotations related to this gene include cytokine activity and CXCR3 chemokine receptor binding. An important paralog of this gene is CXCL10.Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.

Chromosomal Localization:

4q21.1

Expression System:

E. Coli

Accession Number:

BC063122.1

UniProt Accession Number:

Q07325

DNA Source:

OpenBiosystems - MHS1011-9199261

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

5625

Buffers:

PBS without Ca++ and Mg++

Expressed Sequence:

SNATPVVRKGRCSCISTNQGTIHLQSLKDLKQFAPSPSCEKIEIIATLKN
GVQTCLNPDSADVKELIKKWEKQVSQKKKQKNGKKHQKKKVLKVRKSQRS
RQKKTT

Native Sequence:

MKKSGVLFLLGIILLVLIGVQGTPVVRKGRCSCISTNQGTIHLQSLKDLK
QFAPSPSCEKIEIIATLKNGVQTCLNPDSADVKELIKKWEKQVSQKKKQK
NGKKHQKKKVLKVRKSQRSRQKKTT

Calculated Isoelectric Point:

10.34

Molecular Weight:

11997

Last Updated:

09/02/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of CXCL9 (rAg 11056) with molecular weight standards in lane 1
Click image to enlarge

CPTC-CXCL9 PAGE

PAGE of CXCL9 (rAg 11056) with molecular weight standards in lane 1

SOPs

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