Background
Catalog Number:
CPTC-OGG1-1
RRID:
AB_10805279
Target Antigen:
8-Oxoguanine DNA Glycosylase
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Purchase
- DSHB CPTC-OGG1-1
External Links
- Human Protein Atlas CPTC- OGG1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (144.7 KB)
CPTC-OGG1-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-OGG1-1 IHC NCI60
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
IHC Tissue
CPTC-OGG1-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-OGG1-1 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200
Characterization SOP Files
Indirect ELISA
CPTC-OGG1-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Characterization SOP Files
NCI 60 Protein Array
CPTC-OGG1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-OGG1-1 Evaluation by the Human Protein Atlas
Result: Presumed Positive (with additional bands)
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-OGG1-1 Western blot
Result: Positive
Western Blot using CPTC-OGG1-1 as primary Ab against Ag 10783 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
10783
Antigen Name:
8-Oxoguanine DNA Glycosylase
CPTC Name:
CPTC-OGG1
Aliases:
HMMH; HOGG1; MMH; MUTM; OGH1; OTTHUMP00000122619
Function:
This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined.
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
Chromosomal Localization:
3p26.2
Expression System:
E. Coli
Accession Number:
BC000657.2
UniProt Accession Number:
O15527
DNA Source:
HIP : HsCD00041846
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
68660
Buffers:
PBS
Expressed Sequence:
SNAMPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRW
REQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVR
KYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICS
SNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLR
KLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVG
TKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNK
ELGNFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG
Native Sequence:
MPARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQ
SPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYF
QLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNN
NIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLG
LGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKV
ADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELG
NFFRSLWGPYAGWAQAVLFSADLRQCRHAQEPPAKRRKGSKGPEG
Calculated Isoelectric Point:
8.84
Molecular Weight:
38.8
Last Updated:
08/22/2020
Links
- EnsEMBL - EnsEMBL OGG1
- Entrez - Entrez OGG1
- Uniprot - Uniprot O15527
Characterization Data
Gel
Click image to enlarge
CPTC-OGG1 PAGE
PAGE of OGG1 (Ag 10783) in Lanes 2 & 3 with molecular weight standards in lane 1
Characterization SOP Files
SOPs
Don't have Adobe Reader™?
Get it for free at Adobe.com