Catalog Number:
CPTC-GPI-1
RRID:
AB_10573806
Target Antigen:
Glucose Phosphate Isomerase
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:400
Human assay: U-2 OS fixed with PFA, dilution: 1:400
Human assay: U-251 MG fixed with PFA, dilution: 1:400
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA of CPTC-GPI-1
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:250.
Result: Positive
Western Blot using CPTC-GPI-1 as primary Ab against Ag 10078 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GPI-2
RRID:
AB_10571450
Target Antigen:
Glucose Phosphate Isomerase
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA of CPTC-GPI-2
Result: Positive
This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique.
Result: Positive
Protein Array in which CPTC-GPI-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot using CPTC-GPI-2 as primary Ab against Ag 10078 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GPI-3
RRID:
AB_2112658
Target Antigen:
Glucose Phosphate Isomerase
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
09/01/2022
Antigen Recognition(s):
Recombinant Full-length, Endogenous
SOP:
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Indirect ELISA of CPTC-GPI-3
Result: Positive
This file shows the reactivity of the antibody with proteins expressed by the NAPPA technique.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-GPI-3 as primary Ab against Ag 10078 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10078
Antigen Name:
Glucose Phosphate Isomerase
CPTC Name:
CPTC-GPI
Aliases:
GPI; AMF; GNPI; NLK; Neuroleukin; PGI; PHI; SA-36; oxoisomerase; phosphohexomutase; phosphosaccharomutase; EC 5.3.1.9
Function:
This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
Neuroleukin is a neurotrophic factor for spinal and sensory neurons.
Chromosomal Localization:
19q13.1
Expression System:
E. Coli
Accession Number:
BC004982
UniProt Accession Number:
P06744
DNA Source:
HIP : HsCD00003344
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
86985
Buffers:
50mM NH4HC03
Expressed Sequence:
SNAMAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTN
HGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRA
VLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGK
TITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLA
QLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALS
TNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLL
SGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHR
FAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQ
GTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARK
ELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHK
IFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLIN
FIKQQREARVQ
Native Sequence:
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGH
ILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLH
VALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTIT
DVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLN
PESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNT
TKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGA
HWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAA
YFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTK
MIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQ
AAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFV
QGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIK
QQREARVQ
Calculated Isoelectric Point:
8.42
Molecular Weight:
63419
Last Updated:
08/22/2020
PAGE of Ag 10078 (with molecular weight standards in lane 1)
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