Catalog Number:
CPTC-GMNN-1
RRID:
AB_2617263
Target Antigen:
Geminin
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
06/25/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-GMNN-1 as primary Ab against GMNN (Ag 10987) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GMNN-2
RRID:
AB_10805876
Target Antigen:
Geminin
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
09/09/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Tissue Micro-Array(TMA) core of lung cancer showing nuclear staining using Antibody CPTC-GMNN-2. Titer: 1:800
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:200
Human assay: U-2 OS fixed with PFA, dilution: 1:200
Human assay: U-251 MG fixed with PFA, dilution: 1:200
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Western Blot using CPTC-GMNN-2 as primary Ab against GMNN (Ag 10987) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-GMNN-3
Target Antigen:
Geminin
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-GMNN-3 as primary Ab against GMNN (Ag 10987) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
NCI Identification Number:
10987
Antigen Name:
Geminin
CPTC Name:
CPTC-GMNN
Aliases:
Gem; OTTHUMP00000039393; RP3-369A17.3; geminin
Function:
Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Chromosomal Localization:
6p22.2
Expression System:
E. Coli
Accession Number:
BC005185.1
UniProt Accession Number:
O75496
DNA Source:
OB : MHS1011-62496
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
10033
Buffers:
PBS
Expressed Sequence:
SNAMNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAG
LSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQ
YWKEVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEH
VQYMAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEG
TVSSSTDAKPCI
Native Sequence:
MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELSAGLSK
RKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWK
EVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENKELAEVAEHVQY
MAELIERLNGEPLDNFESLDNQEFDSEEETVEDSLVEDSEIGTCAEGTVS
SSTDAKPCI
Calculated Isoelectric Point:
4.93
Molecular Weight:
23.57
Last Updated:
12/11/2009
PAGE of Ag 10987 (with molecular weight standards in lane 1)
No SOPs available.
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