CPTC Reagents Data Portal

Discovery Stage

Background:

The Experimental Design – Unbiased Discovery Working Group of the Clinical Proteomics Technology Assessment for Cancer developed reference materials through several inter-laboratory studies.  The overall goal of these materials is to assess measurement performance of mass-spectrometry based proteomic platforms.  Thus far, these materials are have been used for LC-MS/MS platforms in which the primary analysis is to identify peptides and proteins.  Full descriptions of the materials will be released in a number of forthcoming publications.

The materials fall into two categories.  The first category covers measurement quality assessment materials, such as mixtures and digests composed solely of known amounts of recombinant proteins or synthetic peptides.  The intended use of these materials is for regular assessment of the technical performance of the measurement platform.

The second category of materials includes biological materials such as yeast.  These materials are of increased complexity.  These materials are intended for evaluation of new platforms or comparison of modifications to a platform.  For instance, one may employ a yeast reference material to answer the question, “What is the difference between multiple fractionation methods in terms of the number of low-abundant proteins identified?”

These materials will be available upon publication of the aforementioned manuscripts.  Please send requests for the material to: cancer.proteomics@mail.nih.gov  In your request, please specify, “Discovery reference materials.”