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X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2

Antibody: CPTC-XPNPEP1-2
Background

Catalog Number:

CPTC-XPNPEP1-2

RRID:

AB_2617379

Target Antigen:

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/03/2026

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on colon cancer tissue core using CPTC-XPNPEP1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, lung, prostate, colon and ovarian). Click image to enlarge

CPTC-XPNPEP1-2 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on colon cancer tissue core using CPTC-XPNPEP1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, liver, colon, endometrium, appendix, pancreas, bone marrow, breast, kidney, lung, and placenta) and cancer tissues (breast, lung, prostate, colon and ovarian).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (145.1 KB)

CPTC-ACT Group-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-XPNPEP1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-695).Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-XPNPEP1-2 (plasma)

Result: Positive

Immuno-MRM chromatogram of CPTC-XPNPEP1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-695).Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-XPNPEP1 (peptide 2) Peptide ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-XPNPEP1-2 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-XPNPEP-2 as primary Ab against HEK293T cell lysate containing XPNPEP (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the XPNPEP-2 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-XPNPEP1-2 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-XPNPEP-2 as primary Ab against HEK293T cell lysate containing XPNPEP (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the XPNPEP-2 Ab as the IgG control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-XPNPEP1-2 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-XPNPEP1-2 NCI60 Western blot

Result: Positive

Western Blot using CPTC-XPNPEP1-2 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2
Background

NCI Identification Number:

00021

Antigen Name:

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 2

CPTC Name:

CPTC-XPNPEP1 Peptide 2

Aliases:

X-Prolyl Aminopeptidase 1, Soluble; XPNPEPL; XPNPEPL1; XPNPEP; Aminoacylproline Aminopeptidase; Cytosolic Aminopeptidase P; Soluble Aminopeptidase P; X-Pro Aminopeptidase 1; EC 3.4.11.9; SAMP; X-Prolyl Aminopeptidase; (Aminopeptidase P)-Like; APP1; Aminopeptidase P, Cytosolic; Xaa-Pro Aminopeptidase 1; SAmp

Function:

This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the
N-termini of peptides, such as Arg-Pro-Pro

Chromosomal Localization:

10q25.3

Accession Number:

NP_065116.3

UniProt Accession Number:

Q9NQw7

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TLSLDEVYLIDSGAQYK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1870

Last Updated:

06/11/2013

Links

Characterization Data

SOPs

No SOPs available.

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