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X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 1

Antibody: CPTC-XPNPEP1-1
Background

Catalog Number:

CPTC-XPNPEP1-1

RRID:

AB_2617378

Target Antigen:

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/24/2025

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (136.4 KB)

CPTC-XPNPEP1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-XPNPEP1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-694).Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma. Click image to enlarge

CPTC-XPNPEP1-1 (plasma)

Result: Positive

Immuno-MRM chromatogram of CPTC-XPNPEP1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-694).Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from plasma.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-XPNPEP1 (peptide 1) Peptide ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-XPNPEP1-1 Indirect ELISA

Result: High Binding

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-XPNPEP-1 as primary Ab against HEK293T cell lysate containing XPNPEP (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the XPNPEP-1 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-XPNPEP1-1 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-XPNPEP-1 as primary Ab against HEK293T cell lysate containing XPNPEP (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the XPNPEP-1 Ab as the IgG control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-XPNPEP1-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-XPNPEP1-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-XPNPEP1-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 1
Background

NCI Identification Number:

00020

Antigen Name:

X-prolyl aminopeptidase (aminopeptidase P) 1, soluble Peptide 1

CPTC Name:

CPTC-XPNPEP1 Peptide 1

Aliases:

X-Prolyl Aminopeptidase 1, Soluble; XPNPEPL; XPNPEPL1; XPNPEP; Aminoacylproline Aminopeptidase; Cytosolic Aminopeptidase P; Soluble Aminopeptidase P; X-Pro Aminopeptidase 1; EC 3.4.11.9; SAMP; X-Prolyl Aminopeptidase; (Aminopeptidase P)-Like; APP1; Aminopeptidase P, Cytosolic; Xaa-Pro Aminopeptidase 1; SAmp

Function:

This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.

Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the
N-termini of peptides, such as Arg-Pro-Pro

Chromosomal Localization:

10q25.3

Accession Number:

NP_065116.3

UniProt Accession Number:

Q9NQw7

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GSLTFEPLTLVPIQTK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1760

Last Updated:

06/11/2013

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Characterization Data

SOPs

No SOPs available.

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