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V-Set Domain Containing T Cell Activation Inhibitor 1 Peptide 1

Antibody: CPTC-VTCN1-1
Background

Catalog Number:

CPTC-VTCN1-1

Target Antigen:

V-Set Domain Containing T Cell Activation Inhibitor 1 Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

10/19/2023

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (514.4 KB)

CPTC-VTCN1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (514.4 KB)

CPTC-VTCN1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

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Antibody: CPTC-VTCN1-2
Background

Catalog Number:

CPTC-VTCN1-2

Target Antigen:

V-Set Domain Containing T Cell Activation Inhibitor 1 Peptide 1

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

10/19/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (506.0 KB)

CPTC-VTCN1-2 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (506.0 KB)

CPTC-VTCN1-2 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-VTCN1-2

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-VTCN1-2 as primary antibody against human VTCN1 / B7H4 (25-259, His-tag) recombinant protein (lane 2). Expected molecular weight - 28.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-VTCN1-2 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-VTCN1-2 as primary antibody against human VTCN1 / B7H4 (25-259, His-tag) recombinant protein (lane 2). Expected molecular weight - 28.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-VTCN1-2 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.9 kDa, 18.3 kDa, 9.9 kDa, and 20.7 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-VTCN1-2 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-VTCN1-2 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.9 kDa, 18.3 kDa, 9.9 kDa, and 20.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: V-Set Domain Containing T Cell Activation Inhibitor 1 Peptide 1
Background

NCI Identification Number:

00485

Antigen Name:

V-Set Domain Containing T Cell Activation Inhibitor 1 Peptide 1

CPTC Name:

CPTC-VTCN1 Peptide 1

Aliases:

V-Set Domain Containing T Cell Activation Inhibitor 1; B7-H4; B7H4; V-Set Domain-Containing T-Cell Activation Inhibitor 1; Immune Costimulatory Protein B7-H4; B7 Superfamily Member 1; B7 Family Member, H4; B7 Homolog 4; B7h.5; B7S1; T Cell Costimulatory Molecule B7x; T-Cell Costimulatory Molecule B7x; Protein B7S1; FLJ22418; PRO1291; VCTN1; VTCN1; B7X; B7x

Function:

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1) is a Protein Coding gene. Diseases associated with VTCN1 include Ovarian Cancer. Among its related pathways are Cell adhesion molecules and NF-kappaB Signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding. An important paralog of this gene is BTN3A3.Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.

Chromosomal Localization:

1p13.1-p12

Accession Number:

NP_078902.2

UniProt Accession Number:

Q7Z7D3

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

EGVLGLVHEFK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

03/24/2021

Links

Characterization Data

SOPs

No SOPs available.

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