Catalog Number:
CPTC-VCAM1-1
RRID:
AB_2889970
Target Antigen:
Vascular Cell Adhesion Molecule 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
09/06/2023
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Tissue Micro-Array (TMA) core of ovarian cancer showing cytoplasmic and membranous staining using Antibody CPTC-VCAM1-1. Titer: 1:1000
Result: Negative
Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-VCAM1-1 Ab shows no localization of VCAM1 protein.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western blot using CPTC-VCAM1-1 as primary antibody against human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 78.7 kDa. Molecular weight standards are also included (lane 1).
Result: Positive
Automated western blot using CPTC-VCAM1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 81 kDa. Molecular weight standards are also included (lane 1). Data is positive for HeLa, A549, and MCF7. Data is negative/inconclusive for remaining cell lines.
Result: Positive
Western blot using CPTC-VCAM1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 81 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell line NCI H226. Inconclusive/negative for the other cell lines.
Result: Negative
Single cell western blot using CPTC-VCAM1-1 as a primary antibody against MCF7, NCI H226 and Jurkat cell lysates. VCAM1 protein expression was not detected.
NCI Identification Number:
00401
Antigen Name:
Vascular Cell Adhesion Molecule 1 Peptide 1
CPTC Name:
CPTC-VCAM1 Peptide 1
Aliases:
Vascular Cell Adhesion Molecule 1; Vascular Cell Adhesion Protein 1; CD106 Antigen; INCAM-100; CD106; V-CAM 1; VCAM-1; VCAM1
Function:
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.VCAM1 (Vascular Cell Adhesion Molecule 1) is a Protein Coding gene. Diseases associated with VCAM1 include Viral Encephalitis and Chronic Venous Insufficiency. Among its related pathways are Degradation of the extracellular matrix and Cellular roles of Anthrax toxin. Gene Ontology (GO) annotations related to this gene include integrin binding and primary amine oxidase activity. An important paralog of this gene is MXRA5.Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
Chromosomal Localization:
1p21.2
Accession Number:
NP_001069.1
UniProt Accession Number:
P19320
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
TQIDSPLSGK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1100
Last Updated:
09/02/2020
No SOPs available.
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