Background
Catalog Number:
CPTC-UBE2J1-1
RRID:
AB_2617375
Target Antigen:
ubiquitin-conjugating enzyme E2, J1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
07/28/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-UBE2J1-1
External Links
- Human Protein Atlas CPTC-UBE2J1-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (165.2 KB)
CPTC-UBE2J1-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-UBE2J1-1 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-UBE2J1-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-UBE2J1-1 Western blot
Result: Positive
Western Blot using CPTC-UBE2J1-1 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-UBE2J1-2
RRID:
AB_2617376
Target Antigen:
ubiquitin-conjugating enzyme E2, J1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-UBE2J1-2
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-UBE2J1-2 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CPTC-UBE2J1-2 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-UBE2J1-2. Titer: 1:30000
Characterization SOP Files
Indirect ELISA
CPTC-UBE2J1-2 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-UBE2J1-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-UBE2J1-2 Western blot
Result: Positive
Western Blot using CPTC-UBE2J1-2 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
Catalog Number:
CPTC-UBE2J1-3
RRID:
AB_2617377
Target Antigen:
ubiquitin-conjugating enzyme E2, J1
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
08/25/2021
Antigen Recognition(s):
Recombinant Full-length
Purchase
- DSHB CPTC-UBE2J1-3
Characterization Data [Compare Characterization Data]
IHC HPA
CPTC-UBE2J1-3 Evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Indirect ELISA
CPTC-UBE2J1-3 Indirect ELISA
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Characterization SOP Files
NCI 60 Protein Array
CPTC-UBE2J1-3 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-UBE2J1-3 Western blot
Result: Positive
Western Blot using CPTC-UBE2J1-3 as primary Ab against UBE2J1 (rAg 00013) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Characterization SOP Files
Background
NCI Identification Number:
00013
Antigen Name:
ubiquitin-conjugating enzyme E2, J1
CPTC Name:
CPTC-UBE2J1
Aliases:
NCUBE1; HSPC153; UBC6; Non-canonical ubiquitin-conjugating enzyme 1; Yeast ubiquitin-conjugating enzyme UBC6 homolog E; NCUBE-1; CGI-76; HSPC205; HSU93243; HSUBC6e; Ubc6p; ubiquitin-conjugating enzyme E2, J1, U; EC 6.3.2.19; HsUBC6e
Function:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.
Chromosomal Localization:
6q15
Expression System:
E.coli
Accession Number:
NM_016021
UniProt Accession Number:
Q9Y385
DNA Source:
Imagenes
Immunogen:
Recombinant Full Length Protein
Vector Name:
pNIC28-Bsa4
Extinction Coefficient:
Buffers:
NaP
Expressed Sequence:
MHHHHHHSSGVDLGTENLYFQSMSPAVKRLMKEAAELKDPTDHYHAQPLE
DNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLTANGRFE
VGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTKGEGAIGSLDYTPE
ERRALAKKSQDFCCEGCGSAMKDVLL
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
19659
Last Updated:
04/17/2013
Links
- Entrez - Entrez UBE2J1
- EnsEMBL - EnsEMBL UBE2J1
- Uniprot - Uniprot Q9Y385
Characterization Data
Gel
Click image to enlarge
CPTC-UBE2J1 PAGE
PAGE of UBE2J1 (rAg 00013) with molecular weight standards in lane 1.
Characterization SOP Files
SOPs
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