Catalog Number:
CPTC-UBE2C-3
RRID:
AB_2617368
Target Antigen:
Ubiquitin Conjugating Enzyme E2C Peptide 2
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
10/19/2023
Antigen Recognition(s):
Peptide, Recombinant Full-length
Thematic Panel(s):
DNA Damage Response (DDR) Pathway
SOP:
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Result: Positive
Immuno-MRM chromatogram of CPTC-UBE2C-3 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3260)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Result: Positive
Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.
Result: Positive
Western Blot using CPTC-UBE2C-3 as primary Ab against full-length recombinant Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2C-3 Ab as positive control (lane 3).
NCI Identification Number:
00051
Antigen Name:
Ubiquitin Conjugating Enzyme E2C Peptide 2
CPTC Name:
CPTC-UBE2C Peptide 2
Aliases:
UBCH10; Ubiquitin Carrier Protein C; Ubiquitin-Protein Ligase C; EC 6.3.2.19; Cyclin-Selective Ubiquitin Carrier Protein; DJ447F3.2; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Ubiquitin Carrier Protein E2-C; Ubiquitin-Conjugating Enzyme E2 C; UbcH10
Function:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or
E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the
E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro
catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Chromosomal Localization:
20pq13.12
Accession Number:
NP_008950.1
UniProt Accession Number:
O00762
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LSLEFPSGYPYNAPTVK
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1870
Last Updated:
06/21/2013
No SOPs available.
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