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Ubiquitin Conjugating Enzyme E2C Peptide 1

Antibody: CPTC-UBE2C-2
Background

Catalog Number:

CPTC-UBE2C-2

RRID:

AB_2617367

Target Antigen:

Ubiquitin Conjugating Enzyme E2C Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/18/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (494.1 KB)

CPTC-UBE2C-2 evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-UBE2C-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3259) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-UBE2C-2 IMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-UBE2C-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3259)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (494.1 KB)

CPTC-UBE2C-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2C-2 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2C-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-UBE2C-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2C-2 as primary Ab against full-length recombinant Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2C-2 Ab as positive control (lane 3). Click image to enlarge

CPTC-UBE2C-2 Western blot

Result: Positive

Western Blot using CPTC-UBE2C-2 as primary Ab against full-length recombinant Ag 10213 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2C-2 Ab as positive control (lane 3).


Characterization SOP Files

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Antigen: Ubiquitin Conjugating Enzyme E2C Peptide 1
Background

NCI Identification Number:

00050

Antigen Name:

Ubiquitin Conjugating Enzyme E2C Peptide 1

CPTC Name:

CPTC-UBE2C Peptide 1

Aliases:

UBCH10; Ubiquitin Carrier Protein C; Ubiquitin-Protein Ligase C; EC 6.3.2.19; Cyclin-Selective Ubiquitin Carrier Protein; DJ447F3.2; Mitotic-Specific Ubiquitin-Conjugating Enzyme; Ubiquitin Carrier Protein E2-C; Ubiquitin-Conjugating Enzyme E2 C; UbcH10

Function:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or
E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the
E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene.

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro
catalyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Chromosomal Localization:

20pq13.12

Accession Number:

NP_008950.1

UniProt Accession Number:

O00762

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GISAFPESDNLFK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1430

Last Updated:

06/21/2013

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Characterization Data

SOPs

No SOPs available.

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