Catalog Number:
CPTC-TP53BP1-1
Target Antigen:
Tumor Protein P53 Binding Protein 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
09/06/2023
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous, Phosphorylation
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Positive
Immuno-histochemistry of CPTC-TP53BP1-1 for NCI60 Cell Line Array with titer 1:1250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Result: Positive
Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-TP53BP1-1. Titer: 1:1250
Result: Positive
Immunofluorescence staining of human cell line HeLa with CPTC-TP53BP1-1 Ab shows localization to the nucleus, other.
Result: Positive
Western Blot using CPTC-TP53BP1-1 as primary antibody against recombinant human tumor protein p53 binding protein 1 (TP53BP1), transcript variant 3
protein (lane 2). Also included are molecular weight standards (lane 1).
Result: Negative
Western Blot using CPTC-TP53BP1-1 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).
NCI Identification Number:
00321
Antigen Name:
Tumor Protein P53 Binding Protein 1 Peptide 1
CPTC Name:
CPTC-TP53BP1 Peptide 1
Aliases:
Tumor Protein P53 Binding Protein 1; TP53-Binding Protein 1; P53-Binding Protein 1; P53BP1; 53BP1; Tumor Suppressor P53-Binding Protein 1; Tumor Protein P53-Binding Protein, 1; Tumor Protein 53-Binding Protein, 1; TDRD30; P202
Function:
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.TP53BP1 (Tumor Protein P53 Binding Protein 1) is a Protein Coding gene. Diseases associated with TP53BP1 include Breast Cancer and Riddle Syndrome. Among its related pathways are Metabolism of proteins and DNA Double-Strand Break Repair. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding and p53 binding.Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Chromosomal Localization:
15q15.3
Accession Number:
NP_001135451.1
UniProt Accession Number:
Q12888
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
IDEDGENT(ph)QIEDTEPMS(ph)PVLNSK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2530
Last Updated:
02/19/2020
No SOPs available.
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