Background
Catalog Number:
CPTC-TNFRSF14-1
Target Antigen:
TNF Receptor Superfamily Member 14 Peptide 1
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-TNFRSF14-1
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-TNFRSF14-2
Target Antigen:
TNF Receptor Superfamily Member 14 Peptide 1
Isotype:
IgG2b
Species:
Mouse Recombinant Cloned Antibody
Last Updated:
02/09/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-TNFRSF14-2
Characterization Data [Compare Characterization Data]
Background
Catalog Number:
CPTC-TNFRSF14-3
Target Antigen:
TNF Receptor Superfamily Member 14 Peptide 1
Isotype:
IgG2a
Species:
Mouse Monoclonal Antibody
Last Updated:
02/15/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5985
Purchase
- DSHB CPTC-TNFRSF14-3
Publications
Characterization Data [Compare Characterization Data]
Western Blot - Recombinant Protein or Peptide
CPTC-TNFRSF14-3 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-TNFRSF14-3 as primary antibody against human tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) (TNFRSF14) recombinant protein (lane 2). Expected molecular weight - 26.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-TNFRSF14-3 Western Blot
Result: Negative
Western blot using CPTC-TNFRSF14-3 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.4 kDa and 21.4 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-TNFRSF14-3 Automated Western Blot
Result: Negative
Automated western blot using CPTC-TNFRSF14-3 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), H226 (lane 6), and PBMC (lane 7) whole cell lysates. Expected molecular weight - 30.4 kDa and 21.4 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00480
Antigen Name:
TNF Receptor Superfamily Member 14 Peptide 1
CPTC Name:
CPTC-TNFRSF14 Peptide 1
Aliases:
TNF Receptor Superfamily Member 14; HVEA; HVEM; TR2; Tumor Necrosis Factor Receptor Superfamily, Member 14 (Herpesvirus Entry Mediator); Tumor Necrosis Factor Receptor Superfamily Member 14; Herpes Virus Entry Mediator A; LIGHTR; CD270; ATAR; Tumor Necrosis Factor Receptor Superfamily, Member 14; Tumor Necrosis Factor Receptor-Like Gene2; Tumor Necrosis Factor Receptor-Like 2; Herpesvirus Entry Mediator A; Herpesvirus Entry Mediator; CD40-Like Protein; CD270 Antigen; TNFRSF14; HveA
Function:
This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.TNFRSF14 (TNF Receptor Superfamily Member 14) is a Protein Coding gene. Diseases associated with TNFRSF14 include Herpes Simplex and Nodal Marginal Zone Lymphoma. Among its related pathways are TNF Superfamily - Human Ligand-Receptor Interactions and their Associated Functions and Akt Signaling. Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding and tumor necrosis factor-activated receptor activity. An important paralog of this gene is TNFRSF1B.Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (PubMed:9462508, PubMed:10754304, PubMed:18193050, PubMed:23761635). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed:19915044, PubMed:9153189, PubMed:9162022). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (PubMed:10754304). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed:23761635). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines (By similarity). Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (PubMed:19915044) (By similarity). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells.
Chromosomal Localization:
1p36.32
Accession Number:
NP_003811.2
UniProt Accession Number:
Q92956
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
EDEYPVGSECCPK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1430
Last Updated:
12/17/2020
Links
- EnsEMBL - EnsEMBL TNFRSF14
- Entrez - Entrez TNFRSF14
- Uniprot - Uniprot Q92956
Characterization Data
SOPs
No SOPs available.
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