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Transgelin Peptide 1

Antibody: CPTC-TAGLN-1
Background

Catalog Number:

CPTC-TAGLN-1

RRID:

AB_2617350

Target Antigen:

Transgelin Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

External Links
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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (328.6 KB)

CPTC-TAGLN-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence

CPTC-TAGLN-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF7 cells using CPTC-TAGLN-1 antibody. TAGLN protein expression was not detected.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TAGLN-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-TAGLN-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-TAGLN-1 as primary Ab against full-length recombinant Ag 10361 (lane 2). Also included are molecular wt. standards (lane 1) and the TAGLN-1 Ab as positive control (lane 3). Click image to enlarge

CPTC-TAGLN-1 Western blot

Result: Positive

Western Blot using CPTC-TAGLN-1 as primary Ab against full-length recombinant Ag 10361 (lane 2). Also included are molecular wt. standards (lane 1) and the TAGLN-1 Ab as positive control (lane 3).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-TAGLN-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-TAGLN-1 NCI60 Western blot

Result: Positive

Western Blot using CPTC-TAGLN-1 as primary Ab against lysates from six breast cancer cell lines from the NCI60 cell line collection (lanes 2-7). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

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Antigen: Transgelin Peptide 1
Background

NCI Identification Number:

00042

Antigen Name:

Transgelin Peptide 1

CPTC Name:

CPTC-TAGLN Peptide 1

Aliases:

SM22; WS3-10; SMCC; TAGLN1; Smooth Muscle Protein 22-Alpha; 22 KDa Actin-Binding Protein; SM22-Alpha; DKFZp686P11128; Transgelin Variant 2; Protein WS3-10

Function:

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene.

Actin cross-linking/gelling protein (By similarity). Involved in calcium interactions and contractile
properties of the cell that may contribute to replicative senescence.

Chromosomal Localization:

11q23.2

Accession Number:

NP_001001522.1

UniProt Accession Number:

Q01995

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

AAEDYGVIK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

990

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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