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Signal Transducer And Activator Of Transcription 6 Peptide 1

Antibody: CPTC-STAT6-1
Background

Catalog Number:

CPTC-STAT6-1

Target Antigen:

Signal Transducer And Activator Of Transcription 6 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/19/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

External Links
Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-STAT6-1 Affinity and Kinetics (Bio-Layer Interferometry)

Result: Negative

Affinity and binding kinetics of CPTC-STAT6-1 and full-length human STAT6 recombinant protein were measured using bio-layer interferometry. Full length recombinant protein was amine coupled onto AR2G biosensors. CPTC- STAT6-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without recombinant protein were used as references for background subtraction. All data were analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-STAT6-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-STAT6-1 antibody and human STAT6 recombinant protein using surface plasmon resonance. STAT6 recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-STAT6-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM , 0.25 nM, and 0.0625 nM. Binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (416.8 KB)

CPTC-STAT6-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-STAT6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5965) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-STAT6-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-STAT6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5965)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (416.8 KB)

CPTC-STAT6-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-STAT6-1 as primary antibody against human STAT6 full-length recombinant protein. STAT6 recombinant protein was coated on the plate and was detected using goat anti-rabbit secondary antibody and TMB substrate. Data was fit using a four parameter logistic (4PL) regression model. Click image to enlarge

CPTC-STAT6-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-STAT6-1 as primary antibody against human STAT6 full-length recombinant protein. STAT6 recombinant protein was coated on the plate and was detected using goat anti-rabbit secondary antibody and TMB substrate. Data was fit using a four parameter logistic (4PL) regression model.


Characterization SOP Files

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Antigen: Signal Transducer And Activator Of Transcription 6 Peptide 1
Background

NCI Identification Number:

00456

Antigen Name:

Signal Transducer And Activator Of Transcription 6 Peptide 1

CPTC Name:

CPTC-STAT6 Peptide 1

Aliases:

Signal Transducer And Activator Of Transcription 6; Signal Transducer And Activator Of Transcription 6, Interleukin-4 Induced; IL-4-STAT; D12S1644; Transcription Factor IL-4 STAT; STAT, Interleukin4-Induced; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; STAT6B; STAT6C; STAT6

Function:

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.STAT6 (Signal Transducer And Activator Of Transcription 6) is a Protein Coding gene. Diseases associated with STAT6 include Hemangiopericytoma, Malignant and Nut Allergy. Among its related pathways are Signaling by GPCR and GPCR Pathway. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and sequence-specific DNA binding. An important paralog of this gene is STAT5B.Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.

Chromosomal Localization:

12q13.3

Accession Number:

NP_001171549.1

UniProt Accession Number:

P42226

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GYVPATIK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

880

Last Updated:

10/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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