Background
Catalog Number:
CPTC-STAT1-1
RRID:
AB_2889061
Target Antigen:
Signal Transducer And Activator Of Transcription 1 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/19/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5964
Purchase
- DSHB CPTC-STAT1-1
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (491.6 KB)
CPTC-STAT1-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immuno-MRM
CPTC-STAT1-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-STAT1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5964)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.
Characterization SOP Files
NCI 60 Protein Array
CTPC-STAT1-1 NCI60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-STAT1-1 Western Blot (recombinant protein)
Result: Positive
Western blot using CPTC-STAT1-1 as primary antibody against human signal transducer and activator of transcription 1, (STAT1), transcript variant alpha
recombinant protein (lane 2). Expected molecular weight - 87.2 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-STAT1-1 Automated Western Blot (Cell Lysate)
Result: Negative
Automated western blot using CPTC-STAT1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 87 kDa. Molecular weight standards are also included (lane 1). Data is negative/inconclusive.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-STAT1-1 Western Blot (Cell Lysate)
Result: Negative
Western blot using CPTC-STAT1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 87 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Negative data in all cell lines.
Characterization SOP Files
Background
NCI Identification Number:
00394
Antigen Name:
Signal Transducer And Activator Of Transcription 1 Peptide 1
CPTC Name:
CPTC-STAT1 Peptide 1
Aliases:
Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; ISGF-3; STAT91; CANDF7; IMD31A; IMD31B; IMD31C; STAT1
Function:
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.STAT1 (Signal Transducer And Activator Of Transcription 1) is a Protein Coding gene. Diseases associated with STAT1 include Immunodeficiency 31A and Immunodeficiency 31C. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and Interleukin-11 Signaling Pathway. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and protein homodimerization activity. An important paralog of this gene is STAT4.Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Chromosomal Localization:
2q32.2
Accession Number:
NP_001371809.1
UniProt Accession Number:
P42224
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
YTYEHDPITK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1100
Last Updated:
09/02/2020
Links
- EnsEMBL - EnsEMBL STAT1
- Entrez - Entrez STAT1
- Uniprot - Uniprot P42224
Characterization Data
SOPs
No SOPs available.
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