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SLAM Family Member 8 Peptide 1

Antibody: CPTC-SLAMF8-1
Background

Catalog Number:

CPTC-SLAMF8-1

Target Antigen:

SLAM Family Member 8 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC Tissue

CPTC-SLAMF8-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-SLAMF8-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6220) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-SLAMF8-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-SLAMF8-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6220)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-SLAMF8-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-SLAMF8-1 NCI 60 Protein Array

Result: Presumed Positive

Protein Array in which CPTC-SLAMF8-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-SLAMF8-1 as primary antibody against human SLAM family member 8 (SLAMF8) recombinant protein (lane 2). Expected molecular weight - 31.5 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SLAMF8-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-SLAMF8-1 as primary antibody against human SLAM family member 8 (SLAMF8) recombinant protein (lane 2). Expected molecular weight - 31.5 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-SLAMF8-1 Western Blot (Tissue Lysates)

Result: Negative

Western blot using CPTC-SLAMF8-1 as primary antibody against human breast (2), ovary (3), spleen (4), endometrium (5), and lung (6) tissue lysates. The expected molecular weight is 31.7 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-SLAMF8-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 31.7 kDa. Cytochrome C was used as a loading control. Target is glycosylated. A549 is positive. All other cell lines are negative. Click image to enlarge

CPTC-SLAMF8-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-SLAMF8-1 as primary antibody against human PBMC (2), HeLa (3), Jurkat (4), A549 (5), MCF7 (6) and H226 (7) whole cell lysates. The expected molecular weight is 31.7 kDa. Cytochrome C was used as a loading control. Target is glycosylated. A549 is positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated WB (Simple Western) using CPTC-SLAMF8-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 50.1 KDa. The antibody does not recognize the target in the tested tissue lysates. Click image to enlarge

CPTC-SLAMF8-1 Simple Western (tissue lysates)

Result: Positive

Automated WB (Simple Western) using CPTC-SLAMF8-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 50.1 KDa. The antibody does not recognize the target in the tested tissue lysates.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-SLAMF8-1 Simple Western (cell lysates)

Result: Negative

Automated WB (Simple Western) using CPTC-SLAMF8-1 as primary antibody against the whole lysate of PBMC, HeLa, Jurkat, A549, MCF7 and H226. Expected MW is 31.7 KDa. The antibody does not recognize the target in Jurkat, A549 and H226 lysates. The same cell lysates were probed with an anti-CytC antibody.


Characterization SOP Files

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Antigen: SLAM Family Member 8 Peptide 1
Background

NCI Identification Number:

00393

Antigen Name:

SLAM Family Member 8 Peptide 1

CPTC Name:

CPTC-SLAMF8 Peptide 1

Aliases:

SLAM Family Member 8; BLAME; BCM-Like Membrane Protein; SBBI42; CD353; B Lymphocyte Activator Macrophage Expressed; B-Lymphocyte Activator Macrophage Expressed; CD353 Antigen; SLAMF8

Function:

This gene encodes a member of the CD2 family of cell surface proteins involved in lymphocyte activation. These proteins are characterized by Ig domains. This protein is expressed in lymphoid tissues, and studies of a similar protein in mouse suggest that it may function during B cell lineage commitment. The gene is found in a region of chromosome 1 containing many CD2 genes.SLAMF8 (SLAM Family Member 8) is a Protein Coding gene. Diseases associated with SLAMF8 include Familial Cold Autoinflammatory Syndrome 3. An important paralog of this gene is LY9.May play a role in B-lineage commitment and/or modulation of signaling through the B-cell receptor.

Chromosomal Localization:

1q23.2

Accession Number:

NP_064510.1

UniProt Accession Number:

Q9P0V8

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VGGSVLLVAARPPGFQVR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1980

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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