Background
Catalog Number:
CPTC-RAD50-2
Target Antigen:
RAD50 Double Strand Break Repair Protein Peptide 2
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous, Phosphorylation
External Links
- CPTAC Assay Portal CPTAC-5927 (Phospho)
Purchase
- DSHB CPTC-RAD50-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (304.3 KB)
CPTC-RAD50-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CTPC-RAD50-2 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
Immuno-MRM
CPTC-RAD50-2 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-RAD50-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5927)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-RAD50-2 automated western blot (cell lysate)
Result: Negative
Automated western blot using CPTC-RAD50-2 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.
Characterization SOP Files
Background
NCI Identification Number:
00288
Antigen Name:
RAD50 Double Strand Break Repair Protein Peptide 2
CPTC Name:
CPTC-RAD50 Peptide 2
Aliases:
RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; DNA Repair Protein RAD50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); RAD502; HRad50; HRAD50; NBSLD; RAD50-2; RAD50 Protein; HRAD50
Function:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.RAD50 (RAD50 Double Strand Break Repair Protein) is a Protein Coding gene. Diseases associated with RAD50 include Nijmegen Breakage Syndrome-Like Disorder and Inherited Cancer-Predisposing Syndrome. Among its related pathways are Sensing of DNA Double Strand Breaks and DDX1 as a regulatory component of the Drosha microprocessor.
Chromosomal Localization:
5q31.1
Accession Number:
UniProt Accession Number:
Q92878
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
CYELQQLEGS(ph)SDR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1430
Last Updated:
07/01/2026
Links
- EnsEMBL - EnsEMBL RAD50
- Entrez - Entrez RAD50
- Uniprot - Uniprot Q92878
Characterization Data
SOPs
No SOPs available.
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