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RAD50 Double Strand Break Repair Protein Peptide 1

Antibody: CPTC-RAD50-1
Background

Catalog Number:

CPTC-RAD50-1

RRID:

AB_2888972

Target Antigen:

RAD50 Double Strand Break Repair Protein Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (494.7 KB)

CPTC-RAD50-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAD50-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3286) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-RAD50-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RAD50-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3286)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U2OS shows localization to nuclear bodies. Human assay: THP-1 fixed with PFA, dilution: 1:200 Human assay: U2OS fixed with PFA, dilution: 1:200 Click image to enlarge

CPTC-RAD50-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).
The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U2OS shows localization to nuclear bodies.

Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Single band corresponding to the predicted size in kDa (+/-20%). Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-RAD50-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

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Antigen: RAD50 Double Strand Break Repair Protein Peptide 1
Background

NCI Identification Number:

00098

Antigen Name:

RAD50 Double Strand Break Repair Protein Peptide 1

CPTC Name:

CPTC-RAD50 Peptide 1

Aliases:

RAD50 Homolog; RAD50 Double Strand Break Repair Protein; RAD50 Homolog, Double Strand Break Repair Protein; DNA Repair Protein RAD50; RAD50 (S. Cerevisiae) Homolog; RAD50 Homolog (S. Cerevisiae); EC 3.6.3.27; EC 3.6.1.15; EC 3.6.-.-; EC 3.6.3; RAD502; HRad50; HRAD50; NBSLD

Function:

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.RAD50 (RAD50 Double Strand Break Repair Protein) is a Protein Coding gene. Diseases associated with RAD50 include Nijmegen Breakage Syndrome-Like Disorder and Nijmegen Breakage Syndrome. Among its related pathways are Meiosis and Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA). Gene Ontology (GO) annotations related to this gene include ATPase activity and ATP-dependent DNA helicase activity. An important paralog of this gene is ENSG00000283782.Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation.

Chromosomal Localization:

5q31.1

Accession Number:

NP_005723.2

UniProt Accession Number:

Q92878

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LFDVC*G(pS)QDFESDLDR (pS635)

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1760

Last Updated:

10/22/2019

Links

Characterization Data

SOPs

No SOPs available.

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