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RAD18 Homolog Peptide 1

Antibody: CPTC-RAD18-1
Background

Catalog Number:

CPTC-RAD18-1

RRID:

AB_2617325

Target Antigen:

RAD18 Homolog Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (587.1 KB)

CPTC-RAD18-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-1 antibody with CPTC-RAD18 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-RAD18-1 phospho-peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-1 antibody with CPTC-RAD18 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-1 antibody with CPTC-RAD18 peptide 1 (non-phosphorylated) as the target antigen. Click image to enlarge

CPTC-RAD18-1 non-phospho peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-1 antibody with CPTC-RAD18 peptide 1 (non-phosphorylated) as the target antigen.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (587.1 KB)

CPTC-RAD18-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RAD18-1 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA

CPTC-RAD18-1 Indirect ELISA of non-phospho peptide

Result: Negative

CPTC-RAD18-1 did not react with the non-phosphorylated form of the target peptide in the Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate).


Characterization SOP Files

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Antibody: CPTC-RAD18-2
Background

Catalog Number:

CPTC-RAD18-2

RRID:

AB_2617326

Target Antigen:

RAD18 Homolog Peptide 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/11/2024

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (550.0 KB)

CPTC-RAD18-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-2 antibody with CPTC-RAD18 peptide 1 (phosphorylated) as the target antigen. Click image to enlarge

CPTC-RAD18-2 phospho-peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-2 antibody with CPTC-RAD18 peptide 1 (phosphorylated) as the target antigen.


Characterization SOP Files

  IP
Click to enlarge image Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-2 antibody with CPTC-RAD18 peptide 1 (non-phosphorylated) as the target antigen. Click image to enlarge

CPTC-RAD18-2 non-phospho peptide IPMS

Result: Positive

Immuno-Precipitation Mass Spectrometry using CPTC-RAD18-2 antibody with CPTC-RAD18 peptide 1 (non-phosphorylated) as the target antigen.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (550.0 KB)

CPTC-RAD18-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-RAD18-2 Indirect ELISA

Result: Positive

Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA

CPTC-RAD18-2 Indirect ELISA of non-phospho peptide

Result: Negative

CPTC-RAD18-2 did not react with the non-phosphorylated form of the target peptide in the Indirect peptide ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-RAD18-2 Western Blot - Cell Lysate

Result: Negative

Western Blot using CPTC-RAD18-2 as primary antibody against the cell lysates of SF-268, MDA-MB-231, K-562, HCC-2998, A498, and TK-10. The antibody does not recognize the target protein in the tested lysates.


Characterization SOP Files

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Antibody: CPTC-RAD18-4
Background

Catalog Number:

CPTC-RAD18-4

RRID:

AB_2888969

Target Antigen:

RAD18 Homolog Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/11/2024

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

External Links
Purchase
Publications
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-RAD18-4 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3285) Click image to enlarge

CPTC-RAD18-4 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-RAD18-4 antibody (see CPTAC assay portal for details:
https://assays.cancer.gov/CPTAC-3285)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-RAD18-4 Western Blot - Cell Lysate

Result: Negative

Western Blot using CPTC-RAD18-4 as primary antibody against the cell lysates of SF-268, MDA-MB-231, K-562, HCC-2998, A498, and TK-10. The antibody does not recognize the target protein in the tested lysates.


Characterization SOP Files

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Antigen: RAD18 Homolog Peptide 1
Background

NCI Identification Number:

00084

Antigen Name:

RAD18 Homolog Peptide 1

CPTC Name:

CPTC-RAD18 Peptide 1

Aliases:

RAD18 Homolog (S. Cerevisiae); Postreplication Repair Protein RAD18; RNF73; hHR18; hRAD18; RING Finger Protein 73; E3 Ubiquitin-Protein Ligase RAD18; Postreplication Repair Protein HRAD18p; RAD18, S. Cerevisiae, Homolog; EC 6.3.2.

Function:

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

RAD18 (RAD18 homolog (S. cerevisiae)) is a protein-coding gene. GO annotations related to this gene include damaged DNA binding and ubiquitin protein ligase binding.

E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity

Chromosomal Localization:

3p25-p24

Accession Number:

NP_064550.3

UniProt Accession Number:

Q9NS91

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NHLLQFALE(pS)PAK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1430

Last Updated:

09/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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